Parenteral and oral formulations of benzimidazoles

ABSTRACT

Provided herein are drug delivery systems, such as self-nanoemulsifying drug delivery systems, self-emulsifying drug delivery systems and parenteral microemulsion formulations, suitable for parenteral or oral delivery to a subject. The drug delivery systems may comprise a benzimidazole derivative, e.g., mebendazole, an oil, a surfactant, a cosurfactant and a dipolar aprotic solvent in a microemulsion formulation. Also provided are methods for improving the bioavailability of a benzimidazole derivative during treatment of a pathophysiological condition by using a formulation combining a particular emulsion droplet diameter and ratio of the surfactant:cosurfactant therein, for increasing concentration and retention of a benzimidazole derivative in the lung via a parenterally administerable microemulsion with droplet size of about 35 nm to less than 100 nm and for defining hemolytically safe microemulsions of a benzimidazole derivative during a therapeutic treatment via a parenterally administerable microemulsion with a surfactant:cosurfactant content by weight of about 6% to 48%.

CROSS-REFERENCE TO RELATED APPLICATION

This is a continuation-in-part of nonprovisional application U.S. Ser.No. 10/640,467, filed Aug. 13, 2003 now U.S. Pat. No. 7,419,996.

BACKGROUND OF THE INVENTION

1. Field of the Invention

The present invention relates generally to the fields of pharmacologyand pharmaceutics. More particularly, it concerns pharmaceuticalcompositions that include a benzimidazole, a polyol, and a dipolaraprotic solvent. It also concerns pharmaceutical compositions thatinclude a benzimidazole, an oil, a dipolar aproptic solvent, and asurfactant.

2. Description of the Related Art

The identification of improved methods of drug delivery is crucial inthe treatment of a vast number of human diseases. In particular, eventhough scientific studies might have identified the potential usefulnessof a certain drug in the treatment of a particular condition, treatmentoptions may be limited if proper formulations of the drug to facilitatedelivery of the agent to the diseased tissue are not identified.

One disease wherein pharmaceutics has played a significant role iscancer. Cancer is the second leading cause of death in the UnitedStates. Half of all men and one-third of all women in the United Stateswill develop cancer during their lifetimes. Today, millions of peopleare living with cancer or have had cancer. The sooner a cancer isidentified and treatment initiated, the greater are the chances forsurvival.

Pharmaceutical preparations that are effective against cancer comprisean emerging and expanding area of research and potential commercialdevelopment. Pharmaceutical agents are being developed that can delay orarrest development of cancer, and the development of precancerouslesions into cancers. Precancerous lesions include, for example, lesionsof the breast that can develop into breast cancer, lesions of the skinthat can develop into malignant melanoma or basal cell carcinoma,colonic adenomatous polyps that can develop into colon cancer, cervicaldysplasia that can develop into cervical cancer, premalignant lesions ofthe oropharynx that can develop into head and neck cancer.

The search for drugs useful for treating and preventing neoplasias intheir earliest stages is intensive because chemotherapy and surgeryalone are often ineffective, and current cancer chemotherapy has severeside effects. Such preventive treatment is also potentially useful forrecovered cancer patients who retain a risk of cancer recurrence, andeven for cancer patients who would benefit from compounds thatselectively induce apoptosis in neoplastic, but substantially not innormal cells.

Induction of apoptosis is one mechanism by which pharmaceutical agentscan kill cancer cells. Apoptosis, sometimes referred to as “programmedcell death,” naturally occurs in many tissues in the body. It plays acritical role in tissue homeostasis, that is, it ensures that the numberof new cells produced are correspondingly offset by an equal number ofcells that die. Apoptosis is especially pronounced in self-renewingtissues such as bone marrow, immune cells, gut, and skin.

Standard chemotherapeutics can promote apoptosis not only in cancercells, but also in normal human tissues. These agents often haveparticularly severe effect on tissues where apoptosis is especiallypronounced (e.g., hair, gut, and skin). Thus, standard chemotherapeuticsare inappropriate for cancer prevention, particularly if chronicadministration is indicated.

Benzimidazoles (BZs) are a broad-spectrum class of antihelmintics thatdisplay excellent activity against parasitic nematodes and, to a lesserextent, against cestodes and trematodes. BZs have also been shown to bevery effective antiprotozoal agents that also have antifungal activity.It is currently believed that BZs exert their cytotoxic effects bybinding to the microtubule system and disrupting its functions (Lacey,1988; Friedman and Platzer, 1980). The suggestions that tubulin is atarget for BZs has been supported by the results of drug-binding studiesusing enriched extracts of helminth and mammalian tubulin (Lacey, 1988).Moreover, competitive drug-binding studies using mammalian tubulin haveshown that BZs compete for colchicine binding and inhibit growth ofL1210 murine leukemia cells in vitro (Friedman and Platzer, 1978; Laceyand Watson, 1989). However, BZs are selectively toxic to nematodes whenadministered as antihelmintics but are not toxic to the host. Incontrast, BZs suppress the in vitro polymerization of mammalian tubulin.Differences in both the affinity between the host and parasitemacromolecules for BZ (Russell et al., 1992; Kohler and Bachmann, 1981)and the pharmacokinetics of BZs between the host and the parasite havebeen suggested as responsible for the selective toxicity of BZs(Gottschall et al., 1990) but the actual molecular basis of thisselective toxicity remains unclear.

Mebendazole (MZ), or 5-benzoyl-2-benzimidazole carbamic acid methylester, is a member of the BZ class of compounds. Recently, MZ has beenfound to induce mitotic arrest and apoptosis by depolymerizing tubulinin non-small cell lung cancer cells. (Sasaki et al., 2002). MZ has alsobeen found to elicit a potent antitumor effect on human cancer celllines both in vitro and in vivo (Mukhopadhyay et al., 2002).

MZ was first introduced for the treatment of roundworm infections as aresult of research carried out by Brugmans et al. (1971). It is theprototype of a series of broad-spectrum anthelmintics widely used inboth animals and man (Michiels et al., 1982) as broad-spectrumanthelmintics for animal and human use (Van den Bossche et al., 1982).Related BZ derivatives with anthelmintic properties include albendazoleand flubendazole.

MZ is highly effective in ascariasis, intestinal capillariasis,enterobiasis, trichuriasis, and hookworm (Ancylostoma duodenale andNecator americanus) infection as single or mixed infections. The drug isactive against both larval and adult stages of the nematodes that causethese infections, and it is ovicidal for Ascaris and Trichuris (Keystoneand Murdoch, 1979; Van den Bossche et al., 1982). Immobilization anddeath of susceptible gastrointestinal organisms occurs slowly, andclearance from the gastrointestinal tract may not be complete until afew days after treatment with MZ. Together with albendazole, MZ hasshown some promise in the treatment of hydatid disease (Wilson et al.,1987).

MZ causes selective disappearance of cyoplasmic microtubules in thetegumental and intestinal cells of affected worms. Secretory substancesaccumulate in Golgi areas, secretion of acetylcholinesterase and uptakeof glucose are impaired, and glycogen is depleted. These effects of MZare not noted in host cells. MZ has a high affinity for parasite tubulinin vitro, but it also binds to host tubulin. The biochemical basis forits selective action is thus unclear (see Van den Bossche, 1981; Wattset al., 1982).

The conventional formulation of MZ is a tablet form. Tablets of MZ arepoorly and erratically absorbed, and concentrations of the drug inplasma are low and do not reflect the dosage taken (Witassek et al.,1981). This is because MZ is highly lipophilic, with an aqueoussolubility of less than 1.mu.g/ml. As a result, the conventionalformulations of MZ result in low bioavailability of the drug and erraticabsorption from the gastrointestinal tract. Many other BZs and BZderivatives are also highly lipophilic and erratically absorbed from thegastrointestinal tract.

Therefore, there is a recognized need for improved oral pharmaceuticalcompositions of BZs and BZ derivatives, such as MZ, that result ingreater bioavailability of the drug, are needed to treat systemicdiseases such as cancer and deep-seated parasitic diseases withextraintestinal manifestations. In addition, parenteral formulationsthat result in greater release of BZs and BZ derivatives compared toconventional formulations would also be beneficial in treating thesesystemic conditions. Pharmaceutical compositions such as these wouldresult in greater concentration of the drug in the bloodstream, andconsequentially greater efficacy and therapeutic benefit. The presentinvention fulfills this long-standing need and desire in the art.

SUMMARY OF THE INVENTION

The present invention is based on the development of novel formulationsBZ and BZ derivatives that result in improved drug solubility, improveddrug release from the formulation, and improved bioavailability. Higherconcentration of BZ or BZ derivative in the blood would result ingreater therapeutic effect of this class of agents for use in thetreatment of disorders in which treatment with BZs or BZ derivativeswould be beneficial. For example, in the case of MZ, these diseaseswould include hyperproliferative diseases such as cancer, andextraintestinal and deep-seated parasitic disease such as hydatiddisease.

The inventors have formulated MZ using co-solvency and microemulsionapproaches which have yielded a drug concentration of 1.4-3.5 mg/ml,which is a 1,400-3,500 fold increase in solubility compared toconventional formulations of MZ. The inventors have assessed theformulations of MZ by in vitro USP dissolution, in vivo bioavailabilitystudies in rats, and cell growth inhibition in cell line cultures ofnumerous types of cancer cells. A substantial improvement in release ofthe MZ from the formulations was demonstrated compared to anunformulated suspension, and bioavailability was improved 130 fold. Anenhanced IC₅₀ of MZ from the formulations for cell growth in lung cancercell lines was demonstrated compared to control formulations dissolvedin dimethylsulfoxide and the formulations exhibited favorablecharacteristics in preclinical pharmacokinetic and pharmacodynamicstudies. Therefore, the new BZ formulations are expected to haveenhanced efficacy in the treatment of cancer and otherhyperproliferative diseases.

Certain embodiments of the present invention are pharmaceuticalcompositions that include a BZ, a polyol, and a dipolar aprotic solvent.The definition of pharmaceutical composition is discussed in detailelsewhere in this specification, and encompasses a composition that doesnot produce an adverse, allergic or other untoward reaction whenadministered to an animal, or human, as appropriate.

Any BZ, BZ derivative, or combination of BZs is contemplated for use inthe pharmaceutical compositions of the present invention. One of skillin the art would be familiar with this broad class of agents, and wouldunderstand that the pharmaceutical compositions of the present inventionpertain to use of any member or members of this class of drugs. Forexample, the BZ included in the present pharmaceutical compositions maybe a derivative having the formula:

wherein R³ is selected from the group that includes H, carboxyl (—CO₂H),hydroxyl, amino, chloro, difluormethoxy, benzoyl, phenyl-thio,pyridinyl, propyl-thio, diphenyl, methoxy(methoxy-dimethyl,p-yridinyl)methyl-(sulfonyl), fluorophenylmethyl-2-chloro, propenyl,chloroprophyl or esters (—CO₂R⁴) wherein R⁴ is selected from the groupthat includes alkoxy, haloalkyl, alkenyl, and cycloalkyl, wherein thealkyl groups have from 1-8 carbons, or CH₃CH₂(OCH₂CH₂)_(n)—, orCH₃CH₂CH₂(O CH₂CH₂CH₂)_(n), or (CH₃)₂CH(OCH(CH₃)CH₂)_(n)—, wherein n isfrom 1-3, wherein R¹ is OH, Cl, SH, carbamate or piperidin-4-yl, and R²is hydrogen, .alpha.-methylvinyl, 3-chloropropyl or piperidin-4-yl, orthe pharmaceutically effective organic or inorganic salts thereof, ormixtures thereof.

In certain embodiments of the present invention, the BZ derivative ismethyl 5-benzoylbenzimidazole-2-carbamate (mebendazole, MZ).Alternatively, in other embodiments, the BZ derivative may be methyl5-(phenylthio)-2-benzimidazole carbamate (fenbendazole) or5-methoxy-2-[[(4-methoxy-3,5-dimethyl-2-pyridinyl)methyl]sulfinyl]-1H-ben-zimidazole(omeprazole).

In certain other embodiments, the BZ derivative in the pharmaceuticalcompositions may be

Alternatively, the BZ derivative of the pharmaceutical compositions maybe

In still other embodiments, the pharmaceutical composition includes a BZderivative that is

The pharmaceutical compositions of the present invention contemplate useof any polyol. One of ordinary skill in the art would be familiar withthe class of agents known as polyols, and would understand that any ofthese agents may be included in the pharmaceutical compositions of thepresent invention. For example, the polyol may be polyethylene glycol(PEG) 200, PEG 300, PEG 400, PEG 600, 1,2-propylene diol, glycerol, orethylene glycol. In certain embodiments, the polyol may be PEG 400. Thepharmaceutical compositions of the present invention can include asingle polyol, or more than one polyol.

The pharmaceutical compositions of the present invention include dipolaraprotic solvents. A dipolar aprotic solvent is a solvent with acomparatively high relative dielectric constant, greater than about 15,and a sizable permanent dipole moment, that cannot donate suitablylabile hydrogen atoms to form strong hydrogen bonds. The term issomething of a misnomer, since such solvents are usually not aprotic butprotophilic and, at most, weakly protogenic. One of ordinary skill inthe art would be familiar with the class of agents typically known asdipolar aprotic solvents, and would understand that any of these agentsmay be included in the pharmaceutical compositions of the presentinvention.

For example, the dipolar aprotic solvents include N,N-dimethylacetamide(DMA) or dimethylsulfoxide (DMSO). In certain embodiments, thepharmaceutical compositions include N,N-dimethylacetamide as the dipolaraprotic solvent. In certain other embodiments, the dipolar aproticsolvent is dimethylsulfoxide. In some embodiments, the pharmaceuticalcompositions include more than one dipolar aprotic solvent. For example,the pharmaceutical compositions may include N,N-dimethylacetamide anddimethylsulfoxide.

The pharmaceutical compositions of the present invention contemplate anyratio of polyol to dipolar aprotic solvent by weight. However, incertain embodiments of the present invention, the ratio of polyol todipolar aprotic solvent by weight is 3:1. For example, thepharmaceutical compositions of the present invention may include PEG 400and N,N-dimethylacetamide in a ratio of 3:1 by weight. Alternatively,certain embodiments of the present invention may include PEG 400 anddimethylsulfoxide in a 3:1 ratio by weight.

In some embodiments of the present pharmaceutical compositions, theratio of polyol to dipolar aprotic solvent is 7:1 by weight. Forexample, the pharmaceutical may include PEG 400 and dimethylsulfoxide ina ratio of 7:1 by weight, or PEG 400 and N,N-dimethylacetamide in aratio of 7:1 by weight.

In certain embodiments of the present pharmaceutical compositions, thecomposition includes water. Any amount of water is contemplated by thepresent invention. However, in certain embodiments, the relative ratioof polyol:dipolar aprotic solvent:water is 3:1:1 by weight. For example,some embodiments include a pharmaceutical compositions that may includePEG 400: N,N-dimethylacetamide:water in a 3:1:1 ratio by weight.Alternatively, other embodiments of the present pharmaceuticalcompositions include PEG 400: dimethylsulfoxide:water in a 3:1:1 ratioby weight.

In other embodiments of the present pharmaceutical compositions, therelative ratio of polyol:dipolar aprotic solvent:water is 7:1:2. Forexample, the pharmaceutical composition may include PEG400:dimethylsulfoxide:water in a 7:1:2 ratio by weight.

Additional embodiments of the present invention pertain topharmaceutical compositions that include (1) a BZ; (2) an oil; (3) adipolar aprotic solvent; and (4) a surfactant. Members of the class ofBZ and BZ derivatives are discussed above, and elsewhere in thisspecification. As discussed above, one of skill in the art would befamiliar with this broad class of agents, and would understand that thepharmaceutical compositions of the present invention pertain to use ofany member or combination of members of this class of drugs. Forexample, as discussed above, the BZ derivative may be MZ.

Any oil is contemplated for use in the present invention. One ofordinary skill in the art would be familiar with the wide variety ofoils that are available for inclusion in pharmaceutical compositions.For example, the oil may be super refined corn oil, super refinedcottonseed oil, olive oil, super refined peanut oil, super refinedsoybean oil, Captex 200, Captex 355, Miglyol 812, or Myvacet 9-45. Incertain embodiments of the present pharmaceutical compositions, the oilis Captex 200. In certain other embodiments of the presentpharmaceutical compositions, the oil is Myglyol 812. The pharmaceuticalcompositions of the present invention also contemplate compositionscontaining more than one oil or any combination of oils.

Any surfactant is contemplated for use in the present invention. One ofordinary skill in the art would be familiar with the wide variety ofsurfactants that are available for inclusion in pharmaceuticalcompositions of the present invention. For example, in certainembodiments, the pharmaceutical compositions of the present inventioninclude Tween 80 as the surfactant. In other embodiments, thepharmaceutical compositions include Arelacel 80 as the surfactant. Thepharmaceutical compositions of the present invention may include asingle surfactant, or any combination of surfactants.

Surfactants of any hydrophilic-lipophilic balance (HLB) are contemplatedfor use in the present invention. The HLB of a surfactant is anempirical quantity, on an arbitrary scale, which is a measure of thepolarity of a surfactant or mixture of surfactants. It is a widely knownand used term which would be very familiar to one of ordinary skill inthe art. In general, a high HLB surfactant is a surfactant with an HLBthat is 7 or above 7, and a low HLB surfactant is a surfactant with anHLB below 7. More than one surfactant in the composition may provide forversatility, greater stability, and enhanced drug absorption. Forexample, some embodiments of the present invention include a combinationof Tween 80 and Arelacel 80 as the surfactants.

In certain embodiments of the present pharmaceutical compositions, thesurfactants used are only high HLB surfactants. Other embodiments of thepresent pharmaceutical compositions include only low HLB surfactants.Alternatively, the pharmaceutical compositions of the present inventioninclude a mixture of both high HLB surfactants and low HLB surfactants.

As discussed above, the pharmaceutical compositions of the presentinvention may further be defined as including water. Any amount of wateris contemplated in the present pharmaceutical compositions, as long asany amount of the previously defined components is present.

The pharmaceutical compositions of the present invention may further bedefined as a microemulsion. A microemulsion is herein defined as athermodynamically stable dispersion of one liquid phase into another,stabilized by an interfacial film of surfactant. This dispersion may beeither oil-in-water or water-in-oil. Microemulsions are often clearsolutions, as the droplet diameter may be approximately 100 nanometersor less. However, solutions that are not clear are encompassed withinthe definition of microemulsion used herein, and any droplet diameter iscontemplated in this definition.

Examples of certain embodiments of the present invention includepharmaceutical compositions that include (1) MZ; (2) 10%-60% by weightof an oil; (3) 2%-30% by weight of a dipolar aprotic solvent; (4) 5%-50%by weight of a first surfactant; and (5) 5%-50% by weight of a secondsurfactant.

The pharmaceutical compositions of the present invention contemplate anyconcentration of MZ. For example, in certain embodiments theconcentration of MZ is greater than 1.4 mg/ml. For example, the MZconcentration may be about 1.5 mg/ml, about 1.6 mg/ml, about 1.7 mg/ml,about 1.8 mg/ml, about 1.9 mg.ml, about 2.0 mg/ml, about 2.1 mg/ml,about 2.2 mg/ml, about 2.3 mg/ml, about 2.4 mg/ml, about 2.5 mg/ml,about 2.6 mg/ml, about 2.7 mg/ml, about 2.8 mg/ml, about 2.9 mg/ml,about 3.0 mg/ml, about 3.1 mg/ml, about 3.2 mg/ml, about 3.3 mg/ml,about 3.4 mg/ml, about 3.5 mg/ml, or any range of concentration orincrements of concentration derivable therein.

In other embodiments, the concentration of MZ is 0.3-3.0 mg/ml. Forexample, the concentration of MZ may be about 0.4 mg/ml, about 0.5mg/ml, about 0.6 mg/ml, about 0.7 mg/ml, about 0.8 mg/ml, about 0.9mg/ml, about 1.0 mg/ml, about 1.1 mg/ml, about 1.2 mg/ml, about 1.3mg/ml, about 1.4 mg/ml, about 1.5 mg/ml, about 1.6 mg/ml, about 1.7mg/ml, about 1.8 mg/ml, about 1.9 mg/ml, about 2.0 mg/ml, about 2.1mg/ml, about 2.2 mg/ml, about 2.3 mg/ml, about 2.4 mg/ml, about 2.5mg/ml, about 2.6 mg/ml, about 2.7 mg/ml, about 2.8 mg/ml, about 2.9mg/ml, or any range of concentration or increment of concentrationderivable therein. In other embodiments, the concentration of MZ is 0.5mg/ml to 2.5 mg/ml or 0.3 mg/ml to 3.0 mg/ml.

In certain specific embodiments, the concentration of MZ is about 1.2mg/ml. In other embodiments, the concentration of MZ is about 1.5 mg/ml.In still other embodiments, the concentration of MZ is about 1.7 mg/ml.In additional embodiments, the MZ concentration is about 1.8 mg/ml. Inother embodiments, the MZ concentration is about 1.85 mg/ml. In otherembodiments, the MZ concentration is about 1.9 mg/ml. In furtherembodiments, the MZ concentration is about 2.0 mg/ml. In still furtherembodiments, the MZ concentration is about 2.1 mg/ml. In certain otherembodiments, the concentration of MZ is about 2.3 mg/ml. In furtherembodiments, the MZ concentration is about 2.6 mg/ml.

The concentration of BZ in the pharmaceutical compositions of thepresent invention may be determined by any method known to those ofskill in the art. One of ordinary skill in the art would be familiarwith the range of techniques used to determine concentration of a BZ orBZ derivative in a composition. For example, the concentration of BZ maybe determined by HPLC. In certain embodiments wherein the BZ derivativeis MZ, HPLC may be used to determine the concentration of MZ. Use ofHPLC to determine concentration of MZ in a pharmaceutical composition ofthe present invention is discussed further in the specification below.

In some embodiments of the present invention, the pharmaceuticalcompositions may include water. Any amount of water is contemplated inthe present invention, as long as the other required constituents arepresent. In addition, the pharmaceutical compositions of the presentinvention contemplate the presence of components in addition to thosethat have been delineated. A greater discussion of potential additionaladditives is discussed in the specification below.

Some embodiments of the present pharmaceutical compositions includeTween 80 as the first surfactant and Arelacel 80 as the secondsurfactant. For example, in some embodiments, the pharmaceuticalcompositions include 10%-60% by weight of Captex 200, 3%-30% by weightof N,N-dimethylacetamide, 5%-50% by weight of Tween 80, 5%-50% by weightof Arelacel 80, and 2%-20% by weight of water. In other embodiments, thepharmaceutical composition includes 28.9% by weight of Captex 200, 13.7%by weight of N,N-dimethylacetamide, 28.8% by weight of Tween 80, 28.8%by weight of Arelacel 80, and 13% water.

In certain other embodiments, the pharmaceutical composition includes10%-60% by weight of Captex 200, 2%-20% by weight of dimethylsulfoxide,10%-40% by weight of Tween 80, 10%-40% by weight of Arelacel 80, and10%-20% by weight of water. For example, the pharmaceutical compositionmay include 50.1% by weight of Captex 200, 12.6% by weight ofdimethylsulfoxide, 12.6% by weight of Tween 80, 12.6% by weight ofArelacel 80, and 12.1% by weight of water. The pharmaceuticalcomposition may also include 42.6% by weight of Captex 200, 10.7% byweight of dimethylsulfoxide, 16.1% by weight of Tween 80, 16.1% byweight of Arelacel 80, and 14.5% by weight of water. In other examples,the pharmaceutical compositions includes 38.4% by weight of Captex 200,9.6% by weight of dimethylsulfoxide, 19.2% by weight of Tween 80, 19.2%by weight of Arelacel 80, and 13.6% by weight of water. In furtherexamples, the pharmaceutical composition includes 34.7% by weight ofCaptex 200, 8.7% by weight of dimethylsulfoxide, 21.7% by weight ofTween 80, 21.7% by weight of Arelacel 80, and 13.2% by weight of water.

The pharmaceutical compositions of the present invention may alsoinclude 10%-60% by weight of super refined soybean oil, 2%-20% by weightof dimethylsulfoxide, 10%-40% Tween 80, 10%-40% Arelacel 80, and0.5%-15% by weight of water. For example, the pharmaceutical compositionmay include 56.5% by weight of super refined soybean oil, 14.1% byweight of dimethylsulfoxide, 14.1% by weight of Tween 80, 14.1% byweight of Arelacel 80, and 1.2% by weight of water. In other embodimentsof the present invention, the pharmaceutical composition includes 44.2%by weight of super refined soybean oil, 11.1% by weight ofdimethylsulfoxide, 16.6% by weight of Tween 80, 16.6% by weight ofArelacel 80, and 11.5% by weight of water. In further embodiments, thepharmaceutical composition includes 40.0% by weight of super refinedsoybean oil, 10.0% by weight of dimethylsulfoxide, 20.0% by weight ofTween 80, 20.0% by weight of Arelacel 80, and 10.0% by weight of water.In additional embodiments, the pharmaceutical composition includes 35.7%by weight of super refined soybean oil, 8.9% by weight ofdimethylsulfoxide, 22.3% by weight of Tween 80, 22.3% by weight ofArelacel 80, and 10.8% by weight of water.

Embodiments of the present invention also include pharmaceuticalcompositions that include 10%-60% by weight of Miglyol 812, 2%-20% byweight of dimethylsulfoxide, 10%-40% by weight of Tween 80, 10%-40% byweight of Arelacel 80, and 5%-20% by weight of water. For example, thepharmaceutical composition may include 50.4% by weight of Miglyol 812,12.6% by weight of dimethylsulfoxide, 12.7% by weight of Tween 80, 12.7%by weight of Arelacel 80, and 11.6% by weight of water. In otherexamples, the pharmaceutical composition includes 42.4% by weight ofMiglyol 812, 10.6% by weight of dimethylsulfoxide, 15.9% by weight ofTween 80, 15.9% by weight of Arelacel 80, and 15.2% by weight of water.In other examples, the pharmaceutical compositions include 39.2% byweight of Miglyol 812, 9.8% by weight of dimethylsulfoxide, 19.6% byweight of Tween 80, 19.6% by weight of Arelacel 80, and 11.8% by weightof water. The pharmaceutical composition of the present invention mayalso include 34.7% by weight of Miglyol 812, 8.7% by weight ofdimethylsulfoxide, 21.8% by weight of Tween 80, 21.8% by weight ofArelacel 80, and 13.0% by weight of water.

The present pharmaceutical compositions can be formulated by any methodknown to those of skill in the art. For example, in certain embodimentsthe pharmaceutical composition is formulated for parenteraladministration to a subject. Parenteral administration is defined asadministration by any method other than through the digestive tract ornon-invasive topical or regional routes. For example, parenteraladministration may include administration to a patient intravenously,intradermally, intraarterially, intraperitoneally, intralesionally,intracranially, intraarticularly, intraprostatically, intrapleurally,intratracheally, intravitreally, intratumorally, intramuscularly,subcutaneously, subconjunctivally, intravesicularly, intrapericardially,intraumbilically, by injection, and by infusion.

In other embodiments of the present invention, the pharmaceuticalcomposition is further defined as being suitable for administrationthrough the digestive tract or through non-invasive topical and regionalroutes. For example, the pharmaceutical preparation may be administeredorally. In other embodiments, the pharmaceutical preparation isadministered intranasally, topically, locally, by inhalation, bycontinuous infusion, or by localized perfusion by bathing target cellsdirectly, via a catheter, or via lavage.

Embodiments of the present invention also include drug delivery systemscomprising a benzimidazole derivative formulated in a microemulsion ornanoemulsion for improved release and bioavailability to a cell ortissue in a subject. These emulsions may comprise a self-nanoemulsifyingdrug delivery system or a self-emulsifying drug delivery system. In someembodiments the drug delivery system is defined as comprising the 1)benzimidazole derivative, 2) an oil, 3) a surfactant, 4) a cosurfactant,and 5) a dipolar aprotic solvent. In other embodiments the drug deliverysystem further may include water, particularly at a weight ratio ofabout 50%. In particular embodiments the benzimidazole derivative ismethyl 5-benzoylbenzimidazole-2-carbamate (mebendazole) at a at aconcentration of about 0.9 mg/ml to about 2 mg/ml.

The present drug delivery systems may have a formulation comprising anoil that is Captex 200 or Myglyol, for example, in a weight ratio ofabout 14% to about 42%. The formulation may comprise a surfactant thatis Tween 80 and a cosurfactant that is Transcutol or Capmul MCM., forexample, in individual weight ratios of about 6% to about 48% and with asurfactant:cosurfactant ratio of about 1:0.5 to about 1:1. Theformulation may comprise a dipolar aprotic solvent that isdimethylsulfoxide, for example, in a weight ratio of about 5% to about10%. The system may form an emulsion having a droplet diameter of about35 nm to less than 500 nm. A self-nanoemulsifying drug delivery systemmay comprise droplets with a diameter of about 35 to less than 100 nm. Aself-emulsifying drug delivery system may comprise droplets with adiameter of about 141 nm to less than 500 nm.

In certain embodiments the drug delivery system may comprise methyl5-benzoylbenzimidazole-2-carbamate, about 4% to about 42% by weight ofan oil, about 20% to about 41% by weight each of a surfactant and acosurfactant in about a 1:0.75 to about 1:1 ratio, and about 5% to about10% by weight of dimethylsulfoxide. In these embodiments the methyl5-benzoylbenzimidazole-2-carbamate is at a concentration of about 0.9mg/ml to about 2 mg/ml. In one particular example the drug deliverysystem may comprise about 4% to about 9% by weight of Captex 200 andabout 20% to about 41% by weight each of Tween 80 and Transcutol orCapmul. In this particular example the drug delivery system further maycomprise about 50% by weight of water. Also, the system may be theself-nanoemulsifying drug delivery system where the droplet diameter isabout 35 to about 37 nm. In another particular example the drug deliverysystem may comprise about 42% by weight of Myglyol, about 27% by weightof Tween 80 and Transcutol and about 21% by weight of Capmul. In thisother particular example the system may be a self-emulsifying drugdelivery system where the droplet diameter is about 141-145 nm.

In still other embodiments of the present invention, the drug deliverysystems are further defined as being suitable for improving thebioavailability of a benzimidazole derivative for treatment of apathophysiological condition in a subject. Delivering the benzimidazolederivative to the subject, either parenterally or orally, improves itsbioavailability because an efficacious combination of droplet diameterand surfactant:cosurfactant ratio within the emulsion comprising thesystem increases the half-life of the benzimidazole derivative withinthe tissue. In particular embodiments the droplet diameter is about 34nm to about 143 nm and the surfactant:cosurfactant ratio is about 1:1.Thus, it is contemplated that the pharmaceutical compositions and drugdelivery systems of the present invention are suitable to treat apathophysiological condition such as cancer, for example, a lung cancer,or to treat a pulmonary infection with other antimicrobial agents.

In a related embodiment, the drug delivery systems are defined furtheras being suitable for increasing concentration and retention of abenzimidazole derivative within the lung of a subject in need thereof.Formulating a microemulsion comprising the benzimidazole derivative andother components of the drug delivery system with a droplet size withinthe microemulsion of about 35 nm to less than 100 nm provides forincreased concentration and retention of the benzimidazole derivativewithin the lung upon parenteral administration of the microemulsionthereto.

In still further embodiments, the drug delivery systems are defined asbeing suitable for defining the hemolytic safety of microemulsions of abenzimidazole derivative during a therapeutic treatment regimen for asubject. Formulating a microemulsion comprising the benzimidazolederivative and other components of the drug delivery system with a lowsurfactant:cosurfactant content by weight of about 27% to about 42%results in a reduced hemolytic potential upon parenteral administrationof the microemulsion to the subject.

Other and further aspects, features and advantages of the presentinvention will be apparent from the following description of thepresently preferred embodiments of the invention given for the purposeof disclosure.

BRIEF DESCRIPTION OF THE DRAWINGS

The following drawings form part of the present specification and areincluded to further demonstrate certain aspects of the presentinvention. The invention may be better understood by reference to one ormore of these drawings in combination with the detailed description ofspecific embodiments presented herein.

FIG. 1. Authentic HPLC chromatogram of MZ (0.1 μg/mL, 100 μL-10 ng).

FIG. 2. Representative pseudo-ternary phase diagram of the microemulsionformulations described in Table III-E.

FIG. 3. In vitro release of MZ from microemulsion of formula codes A4and E4.

FIG. 4. Mean pharmacokinetic profile (n=3) of MZ after intravenous bolusadministration of co-solvency formula P7 (3.25 mg/kg) to rats.

FIG. 5. Mean pharmacokinetic profile (n=4) of MZ after oraladministration of microemulsion E4 (4.89 mg/kg) to rats.

FIG. 6. Mean pharmacokinetic profile (n=4) of MZ after oraladministration of suspension formulation (50 mg/kg) to rats.

FIG. 7. Semi-logarithmic pharmacokinetic profiles of MZ afterintravenous bolus administration of co-solvency formulation P7 (3.25mg/kg) to rats.

FIG. 8. Cytotoxicity of MZ E4-microemulsion in three skin cancer celllines (SRB1, A375, 2237).

FIG. 9. Cell killing effects of MZ E4-microemulsion and MZ-DMSO versusplacebos in H1299 cells.

FIGS. 10A-10B. Representative pseudo-ternary phase diagrams for SNEDDswith captex 200/1:1 tween/transcutol/water (FIG. 10A) and myglyol/1:1tween/transcutol/water (FIG. 10B) combinations.

FIGS. 11A-11B. Comparative release profiles of mebendazole from SNEDDs (

), SEDDS (

), cosolvent formulations (

), and unformulated suspensions (

) (FIG. 11A) and from unformulated suspension and unformatted drug inmedia with placebo SNEDDs (

) (FIG. 11B).

FIGS. 12A-12G. Mean plasma-concentration time profiles of mebendazole inSprague-Dawley rats after i.v. bolus of parenteral cosolvent formulation(FIG. 12A), after oral administration of SNEDDS (FIG. 12B), SEDDS (FIG.12C) and unformulated suspension (FIG. 12D) and after i.v. bolus ofparenteral microemulsions PM1 (FIG. 12E) and PM2 (FIG. 12F). FIG. 12G isthe determination of hemolytic potential of PM1 formulation.

FIG. 13. Plasma concentration profiles of mebendazole from cosolvent (♦)and parenteral microemulsions PM1 (▪) and PM2 (▴) formulations in mice.

FIGS. 14A-14C. Biodistributions for cosolvent (FIG. 14A), PM1 (FIG. 14B)and PM2 (FIG. 14C) formulations in mice.

FIGS. 15A-15C. Comparison of mebendazole concentrations in organs at 5min (FIG. 15A), 2 hr (FIG. 15B) and 4 hr (FIG. 15C) after i.v. injectionof cosolvent, PM1 (37 nm), and PM2 (478 nm) in mice (n=4-5).

FIG. 16. Schematic Representation of a Three-Compartmental Model forLinking the Mbz Concentrations in Lung and in Plasma.

FIGS. 17A-17F. Three-compartmental model fitting of semi-log plot ofmean plasma concentrations and mean lung concentrations of Mbz afteri.v. administration of cosolvent (FIGS. 17A-17B), PM1 (FIGS. 17C-17D)and PM2 (FIGS. 17E-17F) formulations.

FIGS. 18A-18F. Observed vs. predicted plasma and lung concentrations forcosolvent (FIGS. 18A-18B), PM1 (FIGS. 18C-18D), and PM2 (FIGS.18E-18F),for the three-compartment model.

FIGS. 19A-19F. Allometric relationships between Mbz clearance (FIGS.19A-19C) and Mbz Vss (FIGS. 19D-19F) and body weight (log-log) forcosolvent (FIGS. 19A, 19D), PM1 (FIGS. 19B, 19E) and PM2 (FIGS. 19C,19F).

FIGS. 20A-20F. Allometric relationships between Mbz t_(1/2, alpha)(FIGS. 20A-20C) and Mbz t_(1/2, beta) (FIGS. 20D-20F) and body weight(log-log) for cosolvent (FIGS. 20A, 20D), PM1 (FIGS. 20B, 20E) and PM2(FIGS. 20C, 20F).

DETAILED DESCRIPTION OF THE INVENTION

As used herein, the term “a” or “an”, when used in conjunction with theterm “comprising” in the claims and/or the specification, may refer to“one,” but it is also consistent with the meaning of “one or more,” “atleast one,” and “one or more than one.” some embodiments of theinvention may consist of or consist essentially of one or more elements,method steps, and/or methods of the invention. It is contemplated thatany method or composition described herein can be implemented withrespect to any other method or composition described herein.

As used herein, the term “or” in the claims refers to “and/or” unlessexplicitly indicated to refer to alternatives only or the alternativesare mutually exclusive, although the disclosure supports a definitionthat refers to only alternatives and “and/or.”

As used herein, the terms “pharmaceutical,” “pharmaceutically,” or“pharmacologically acceptable” refer to molecular entities andcompositions that do not produce an adverse, allergic or other untowardreaction when administered to an animal, or human, as appropriate. Theterm “pharmaceutical” includes any and all solvents, dispersion media,coatings, antibacterial and antifungal agents, isotonic and absorptiondelaying agents and the like. The use of such media and agents forpharmaceutical active substances is well known in the art. Exceptinsofar as any conventional media or agent is incompatible with theactive ingredients, its use in the therapeutic compositions iscontemplated. Supplementary active ingredients, such as otheranti-cancer agents or anthelmintics, can also be incorporated into thecompositions.

As used herein, the term “hemolytic potential” or “H10%” refers to theratio of a microemulsion formulation to blood for which 90% of the bloodcells are viable.

As used herein, the term “subject” refers to any recipient of abenzimidazole derivative via the pharmaceutical compositions or drugdelivery systems described herein.

The present invention seeks to exploit the inventors' discovery byproviding pharmaceutical compositions of BZs and BZ derivatives thathave improved and predictable bioavailabilities. BZs and BZ derivativesoften have limited and erratic oral bioavailability due to poorsolubility of the compound in aqueous medium. As a result, these drugscannot be efficiently delivered in convention dosage forms. For example,MZ has a solubility in aqueous medium of less that 1 μg/ml. Theinventors have discovered certain oral and parenteral formulations ofBZs and BZ derivatives, such as MZ, using co-solvency and microemulsionapproaches, respectively. The parenteral formulation of MZ was developedto circumvent the drawback of extensive first-pass metabolism of MZknown to be associated with oral administration. These formulations canbe applied for the therapy of diseases wherein BZs and BZ derivativesformulated for increased bioavailability may be efficacious, such ashyperproliferative diseases, neoplastic disease and extraintestinalparasitic diseases.

A. Benzimidazoles and Derivatives of Benzimidazoles

Benzimidazoles (BZs) are broad-spectrum antihelmintics that displayexcellent activity against parasitic nematodes and, to a lesser extent,against cestodes and trematodes. BZs have also been shown to be veryeffective antiprotozoal agents and to have antifungal activity. It iscurrently believed that BZs exert their cytotoxic effects by binding tothe microtubule system and disrupting its functions (Lacey, 1988;Friedman and Platzer, 1980). The suggestion that tubulin is a target forBZs has been supported by the results of drug-binding studies usingenriched extracts of helminth and mammalian tubulin (Lacey, 1988).Moreover, competitive drug-binding studies using mammalian tubulin haveshown that BZs compete for colchicine binding and inhibit growth ofL1210 murine leukemia cells in vitro (Friedman and Platzer, 1978; Laceyand Watson, 1989). However, BZs are selectively toxic to nematodes whenadministered as antihelmintics but are not toxic to the host. Incontrast, BZs suppress the in vitro polymerization of mammalian tubulin.Differences in both the affinity between the host and parasitemacromolecules for BZ (Russell et al., 1992; Kohler and Bachmann, 1981)and the pharmacokinetics of BZs between the host and the parasite havebeen suggested as responsible for the selective toxicity of BZs(Gottschall et al., 1990) but the actual molecular basis of thisselective toxicity remains unclear.

Benzimidazoles and derivatives of benzimidizoles are defined herein tohave the formula:

wherein R is selected from the group consisting of H, carboxyl (—CO₂H),hydroxyl, amino or esters (—CO₂R′) wherein R′ is selected from the groupconsisting of alkoxy, haloalkyl, alkenyl, and cycloalkyl wherein thealkyl groups have 1-8 carbons or CH₃CH₂(OCH₂CH₂)_(n)— orCH₃CH₂CH₂(OCH₂CH₂CH₂)_(n) or (CH₃)₂CH(OCH(CH₃)CH₂)_(n) where n is from1-3 and the pharmaceutically acceptable organic or inorganic additionsalts thereof; wherein R³ is selected from the group consisting of H,carboxyl (—CO₂H), hydroxyl, amino, chloro, difluormethoxy, benzoyl,phenyl-thio, pyridinyl, propyl-thio, diphenyl,methoxy(methoxy-dimethyl,pyridinyl)methyl-(sulfonyl),fluorophenylmethyl-2-chloro, propenyl, chloroprophyl or esters (—CO₂R⁴)wherein R⁴ is selected from the group consisting of alkoxy, haloalkyl,alkenyl, and cycloalkyl, wherein the alkyl groups have from 1-8 carbons,or CH₃CH₂(OCH₂CH₂)_(n)— or CH₃CH₂CH₂(OCH₂CH₂CH₂)_(n)— or(CH₃)₂CH(OCH(CH₃)CH₂)_(n)—, wherein n is from 1-3, wherein R¹ is OH, Cl,SH, carbamate or piperidin-4-yl, and R² is hydrogen, alpha.-methylvinyl,3-chloropropyl or piperidin-4-yl, or the pharmaceutically effectiveorganic or inorganic salts thereof, or mixtures thereof. For R⁴, thepreferred alkyl groups are straight chain, the preferred the halogen issubstituted on the terminal carbon, the preferred halogen is chlorine,the preferred cycloalkyl groups are those having 3-6 carbon atoms, andthe cycloalkyl groups also include those which are substituted on analkyl chain, 2-cyclopropylethyl, cyclopropylmethyl, 2-cyclopropylpropylor 2-cyclopropylpropyl or cyclohexylmethyl.

BZs have some anti-tumor growth properties in vitro (WO 98/513304; WO98/32440). The efficacy of BZs in vivo as an anti-tumor treatment hasbeen limited to tumors already in regression following chemotherapeutictreatment.

Alternative benzimidazoles are: fenbendazole, albendazole, albendazolesulfone, oxibendazole, rycobendazole, thiabendazole, oxfendazole,flubendazole and carbendazim. Alternative anti-helminthic drugs are theimidazoles: niridazole and levimasole, the piperazines: piperazine anddiethylcarbamazine, the isothiocyanates: amoscanate and CGP 6140. Inaddition, suramin, ivermectin, hycanthone, metrifonate, oxamniquine andpraziquantel are anti-helminthic drugs.

B. Treatment of Cancers Using Benzimidazoles

There is evidence, as discussed above, that BZs or BZ derivatives may beuseful in the treatment of hyperproliferative diseases, such as cancer.In particular, the use of BZs in the treatment of cancer is discussed indetail in U.S. patent application Ser. No. 10/043,877, which isspecifically incorporated by reference herein. In addition, MZ has alsobeen found to elicit a potent antitumor effect on human cancer celllines both in vitro and in vivo (Mukhopadhyay et al., 2002; Liang etal., 2002). Mukhopadhyay et al., 2002, and Liang et al., 2002, arespecifically incorporated by reference herein.

Of particular interest are patients that have wild-type tumor suppressor(e.g., p53) function. The tumor suppressor status of the tumor cells canbe determined using any conventional methods, examples of which aredescribed below. Patients may, but need not, have received previouschemo-, radio-, or gene therapies. Optimally, patients will haveadequate bone marrow function (defined as peripheral absolutegranulocyte count of >2000/mm³ and platelet count of 100,000/mm³),adequate liver function (bilirubin ≦1.5 mg/dl) and adequate renalfunction (creatinine <1.5 mg/dl).

Patients with cancer may be treated with a pharmaceutically acceptableform of BZ or a functional analog thereof. This administration could bein the form of, for example, an intratumoral injection, or indeed, anyother method of application that is routinely used and well known to oneof skill in the art, e.g., oral, systemic, local, regional. A biopsy ofthe lesions to be injected may be performed and the tissue stored forimmunohistochemistry analyses.

The dose of BZ typically will be reconstituted into a pharmaceuticallyacceptable form immediately prior to administration. The dose of BZ willbe determined depending on the clinical condition to be treated. Forexample, if the disease is cancer, the starting dose will beapproximately 0.1 to 1 mg BZ/kg body weight. Of course, this may varydepending on the disease to be treated, the size of the tumor, the rateat which the tumor is growing, etc.

C. Pharmaceutical Compositions and Routes of Administration

1. Overview

The objective of the present invention was to develop both parenteraland oral formulations of BZ and BZ derivatives, such as MZ, in order toachieve improved and predictable bioavailabilities. The invention stemsfrom the desire to overcome the problem of limited and erratic oralbioavailability due to poor solubility in aqueous medium of many BZs andBZ derivatives. The parenteral and oral formulations of BZs described ingreater detail below were developed using co-solvency andself-emulsifying/microemulsion approaches, respectively. The parenteralformulation of BZ were developed to circumvent the drawback of theextensive first-pass metabolism of MZ and other BZs known to beassociated with oral administration.

The inventors have investigated the solubility of MZ in varioussolvents, such as N,N-dimethylacetamide (DMA), DMSO, and polyethyleneglycol-400 (PEG), as discussed in greater detail in the examples below.As primary solvents, the solvents would be miscible in secondarysolvents, examples of which are normal saline, dextrose in water (5% or10%), and water. These solvents are examples of vehicles in which BZssuch as MZ could be suitably solubilized, yet be safe for humanadministration, alone or in combinations with other drugs. Thesolubility of BZ in individual solvent vehicles is shown in Table Ibelow.

Virtually no MZ is absorbed through the intestinal tract after oraladministration, making it impossible to even investigate its use as anoral antimicrobial against systemic infections. Parenteraladministration would therefore be the logical approach to evaluate MZand other BZs as therapy for deep-seated, systemic fungal infections andalso in the treatment of cancer and other hyperproliferative diseases.

2. Effective Amount of BZs and BZ Derivatives

Pharmaceutical compositions of the present invention will include aneffective amount of a BZ or a BZ derivative or a mixture thereof that isclinically determined to be useful in the treatment of the particulardisease under consideration. For example, an effective amount of a BZ ora BZ derivative in a patient with cancer may be an amount that promotesexpression of wild-type tumor suppressor genes, and/or inhibitsangiogenesis. Alternatively, an effective amount of a BZ or BZderivative or mixture thereof may be an amount that promotes death ofparasites and/or regression of clinical findings associated with theinfection. Such compositions will generally be dissolved or dispersed ina pharmaceutically acceptable carrier.

In cancer patients, the access to parenteral BZs such as MZ will beparticularly important, since their intestinal absorption is oftenperturbed after chemotherapy, aggravating the already erratic intestinalabsorption of various medications. The parenteral route will also makeit possible to circumvent unpredictable first-pass metabolic effects inthe liver, well known to alter the bioavailability of numerouspharmacologically active agents after oral dosing. Further, theavailability of MZ for effective and reliable systemic administrationwill for the first time make it possible to clinically compare theactivity of MZ against other treatments for cancer and deep-seatedfungal infections.

3. Oral Administration

In addition to the compounds formulated for parenteral administration,such as those for intravenous, subcutaneous, or intramuscular injection,other pharmaceutically acceptable forms include, solutions suitable fororal administration. The term “oral administration” includes any form ofadministration of drug through the oral cavity and into thegastrointestinal tract. The pharmaceutical compositions of the presentinvention may be formulated for delivery to a subject by any means. Forexample, the compositions may be formulated as mouthwashes, mouthrinses,solutions for administration through a nasogastric tube, and the like.

4. Compositions Suitable for Parenteral Administration

As discussed in the summary of the invention, the active compounds ofthe present invention will often be formulated for parenteraladministration, e.g., formulated for injection by any route other thanthrough the digestive tract, such as via the intravenous, intramuscular,subcutaneous, or even intraperitoneal routes. The definition ofparenteral administration is discussed further in the summary of theinvention. Typically, the compositions can be prepared as injectables,either as liquid solutions or suspensions for any parenteral butintravenous routes; solid forms suitable for using to prepare solutionsor suspensions upon the addition of a liquid prior to injection can alsobe prepared; and the preparations can also be emulsified.

5. Formulation Principles

Solutions of the active compounds as free base or pharmacologicallyacceptable salts can be prepared in water suitable for mixture with asurfactant, such as hydroxypropylcellulose or polysorbate. Dispersionscan also be prepared in glycerol, liquid polyethylene glycols, andmixtures thereof and in oils. Under ordinary conditions of storage anduse, these preparations contain a preservative to prevent the growth ofmicroorganisms.

The pharmaceutical forms suitable for injectable use include, forexample, sterile aqueous solutions or dispersions, and formulationsincluding sesame oil, peanut oil or aqueous propylene glycol or othersolvent as discussed in the examples below. In all cases the form mustbe sterile and must be fluid to the extent that easy syringabilityexists. It must be chemically and physically stable under the conditionsof manufacture and storage and must be preserved against thecontaminating action of microorganisms, such as bacteria and fungi.

The active compounds may be formulated into a composition in a neutralor salt form. Pharmaceutically acceptable salts, include the acidaddition salts (formed with the free amino groups of the protein) andwhich are formed with inorganic acids such as, for example, hydrochloricor phosphoric acids, or such organic acids as acetic, oxalic, tartaric,mandelic, and the like. Salts formed with the free carboxyl groups canalso be derived from inorganic bases such as, for example, sodium,potassium, ammonium, calcium, or ferric hydroxides, and such organicbases as isopropylamine, trimethylamine, histidine, procaine and thelike.

The carrier also can be a solvent or dispersion medium containing, forexample, water, ethanol, polyol (for example, glycerol, propyleneglycol, and liquid polyethylene glycol, and the like), suitable mixturesthereof, and vegetable oils. Preparation of cosolvency formulations andmicroemulsion formulations of BZs or BZ derivatives is discussed ingreater detail in the examples below.

The proper fluidity can be maintained, for example, by the maintenanceof the required particle size in the case of dispersion and by the useof surfactants. The prevention of the action of microorganisms can bebrought about by various antibacterial and antifungal agents, forexample, parabens, chlorobutanol, phenol, sorbic acid, thimerosal, andthe like. In many cases, it will be preferable to include isotonicagents, for example, sugars or sodium chloride. Prolonged absorption ofthe injectable compositions can be brought about by the use in thecompositions of agents delaying absorption, for example, gelatin.

Sterile injectable solutions are prepared by incorporating the activecompounds in the required amount in the appropriate solvent with variousof the other ingredients enumerated above, as required, followed byfiltered sterilization. Generally, dispersions are prepared byincorporating the various sterilized active ingredients into a sterilevehicle which contains the basic dispersion medium and the requiredother ingredients from those enumerated above.

In certain cases, the therapeutic formulations of the invention couldalso be prepared in forms suitable for topical administration, such asin gels, creams, and lotions. These forms may be used for treatingskin-associated diseases, such as various sarcomas.

Upon formulation, solutions will be administered in a manner compatiblewith the dosage formulation and in such amount as is therapeuticallyeffective. The formulations are easily administered in a variety ofdosage forms, such as the type of injectable solutions described above,with even drug release capsules and the like being employable.

For parenteral administration in aqueous solution, for example, thesolution should be suitably buffered if necessary. These particularsolutions are especially suitable for intravenous, intramuscular,subcutaneous and intraperitoneal administration. Some variation indosage will necessarily occur depending on the condition of the subjectbeing treated. The person responsible for administration will, in anyevent, determine the appropriate dose for the individual subject.

6. Formulation of BZs and BZ Derivatives that are Sparingly Soluble inWater: Co-Solvency Systems

Many BZs are only sparingly soluble in aqueous solutions. As discussedabove, it is therefore an aspect of the current invention that the BZcompound be in a formulation which allows for an increasedsolubilization of the BZ or for a more effective dispersion.

Generation of some of the pharmaceutical compositions of the presentinvention are based on the principle of cosolvency (Spiegel andNoseworthy, 1963; Yalkowsky and Roseman, 1981). The examples show thatpharmaceutical compositions of MZ can be generated without destroyingits anti-cancer properties. Further, the animal studies provide evidencethat the preferred vehicles are nontoxic and safe for administration andshould be acceptable for human administration in the proposedconcentrations and total doses to be utilized; indeed, DMA, DMSO, and PGhave been used for solubilization of various pharmacologically activeagents used in man (NIH Pub. 84-2141, 1984; Weiss et al., 1962; Kim,1988). The parenteral administration of PEG has been studied in detailin a simian model (Lockard et al., 1979), and PEG has subsequently beenused clinically as a (covalently bound) carrier of L-asparaginase in thetreatment of lymphocytic leukemia and lymphoma (Keating et al., 1993).DMSO is also extensively used as a cryoprotective agent forlow-temperature storage of human bone marrow and peripheral bloodderived hematopoietic stem cell preparations to be used fortransplantation after high-dose chemotherapy (McGann, 1978; Gorin, 1986;Davis and Rowley, 1990; Gorin, 1992). No serious adverse effects havebeen experienced from the use of these vehicles. The clinical use ofnormal saline, dextrose in water (5-70%), and aqueous lipid emulsion arewell established means to alter the fluid and electrolyte balance and tosupply parenteral nutrition. Normal saline and dextrose in water areextensively used to dilute various medications for parenteral use.

7. Formulation of BZs and BZ Derivatives that are Sparingly Soluble inWater: Microemulsion Systems

The preparation and use of microemulsions in the formulation of drugs,proteins, and the like are known in the art. A microemulsion is hereindefined as a thermodynamically stable dispersion of one liquid phaseinto another, stabilized by an interfacial film of surfactant. Thisdispersion may be either oil-in-water or water-in-oil. Microemulsionsare often clear solutions, as the droplet diameter may be approximately100 nanometers or less. However, solutions that are not clear areencompassed within the definition of microemulsion used herein, and anydroplet diameter is contemplated in this definition.

Surfactants can be added to the aqueous solution to increase solubilityand stability of a solution or dispersion. Surfactants, discussed ingreater detail in the summary of the invention, are organic moleculeswhich contain both hydrophilic and hydrophobic ends. The hydrophilicend, which is either polar or ionic, dissolves readily in water. Thehydrophobic, or non-polar, end, however, does not dissolve in water andwill move as far away from water as possible. When a small concentrationof surfactant is added to water, the hydrophobic end will immediatelyrise to the surface or orient towards a less polar group. The additionof surfactants in a BZ solution increase the solubility and/or stabilityof the BZ-surfactant solution relative to the BZ alone.

D. Methods of Measuring Concentration of Drugs in a Composition

Following preparation of the pharmaceutical compositions of the presentinvention, it may be desirable to quantify the amount of BZ or BZderivative in the pharmaceutical composition. Methods of measuringconcentration of a drug in a composition include numerous techniquesthat are well-known to those of skill in the art. Selected examplesinclude chromatographic techniques. There are many kinds ofchromatography which may be used in the present invention: drug-specificassays, adsorption, partition, ion-exchange and molecular sieve, andmany specialized techniques for using them including column, paper,thin-layer chromatography, gas chromatography, and high performanceliquid chromatography (HPLC). One of ordinary skill in the art would befamiliar with these and other related techniques.

The following examples are included to demonstrate preferred embodimentsof the invention. It should be appreciated by those of skill in the artthat the techniques disclosed in the examples which follow representtechniques discovered by the inventors to function well in the practiceof the invention, and thus can be considered to constitute preferredmodes for its practice. However, those of skill in the art should, inlight of the present disclosure, appreciate that many changes can bemade in the specific embodiments which are disclosed and still obtain alike or similar result without departing from the spirit and scope ofthe invention.

EXAMPLE 1

Materials and Methods

Materials

Mebendazole (MZ, Lot 90K1145), polyethylene glycol (PEG) 400 (Lot121H0052), N,N-demethylacetamide (DMA) (Lot 129H3498), anddimethylsulfoxide (DMSO) (Lot 94H0358) were purchased from SigmaChemical Co. (St. Louis, Mo.). Propylene glycol (Lot 940629) and oliveoil (Lot CAS8001-25-0) were obtained from Wood Scientific, Inc (Houston,Tex.). Triacetin was purchased from Lancaster Synthesis, Inc. (Windham,N.H.). Super refined cottonseed oil (Lot GAB-217NP), super refinedpeanut oil (Lot BSS-429NP) and super refined soybean oil (Lot SB4-393NP)were supplied by Croda (Mill Hall, Pa.). Captex 200 (C8/C10 diesters ofpropylene glycol, Lot 10505-6) and Captex 355 (C8/C10 triglycerides fromcoconut oil) were provided by Abitec Corporation (Janesville, Wis.).Miglyol 812 (C8/C10 triglycerides from coconut oil, Lot 1995050866) andMyvacet 9-45 (distilled acetylated monoglycerides; Lot 960225) wereobtained from Eastman Chemical Products. Tween 80 (polyoxytheylene [20]sorbitan monooleate; Lot 32332) and Arelacel 80 (sorbitan monooleate NF,NLB=4.3, Lot 27255) were supplied by ICI Americans Inc. (Wilmington,Del.). Cremophor RH 40 (PEG-40 hydrogenated castor oil, HLB=13.5) wasfrom BASF (Parsippany, N.J.).

HPLC Assay of MZ

An HPLC assay was developed to quantify MZ in the formulations and inthe release samples. The assay used a reversed-phase .mu.Bondapak C₁₈column (300×3.9 mm, 10 μm, Waters Corporation, Milford, Mass.) precededby a C.sub.18 guard column. The mobile phase, which was filtered anddegassed prior to use, consisted of acetonitrile/0.05 M potassiummonobasic phosphate, pH 6.5 (40:60, v/v). Prednisone was used as theinternal standard (IS). The flow rate was 1.2 ml/min. The solventdelivery system was a ConstaMetric 3500 LDC Analytical Model system. MZand IS were detected with a SpectroMonitor 3200 programmablemultiwavelength UV detector set at 254 nm. Peak area ratios measured bya Milton Ray CI-4100 data module integrator (LDC Analytical) were usedto construct standard curves and to determine the concentrations of MZin the samples. The assay was later modified for the quantification ofMZ in blood samples, as described below.

Evaluation of MZ Solubility in Various Solvent Media

The solubility of MZ in aqueous and in fourteen other solvent media,including six pharmaceutical solvents (methanol, PEG 400, propyleneglycol, triacetin, DMA, and DMSO) and eight naturally occurring andsynthetic oils (super refined cottonseed oil, olive oil, super refinedpeanut oil, super refined soybean oil, Captex 200, Captex 355, Myglyol812, and Myvacet 9-45), were evaluated. The solubility of MZ in eachsolvent medium was determined by adding excessive amount of MZ to themedium, followed by shaking at room temperature for at least 48 hours.After the equilibrium was reached, an aliquot was withdrawn and filteredthrough a membrane with a pore size of 0.45 μm. The clear filtrate wasdiluted with an appropriate volume of DMA and analyzed by the developedHPLC assay.

Development of Parenteral Formulations Using Co-Solvency Approach

One hundred milligrams of MZ were dissolved in 11 ml of DMA by warmingin a 60.degree. water bath. The solution was then mixed with PEG 400 atratios of 1:1 to 1:7 (v/v). A final portion of water was added to obtaina final co-solvent formulation with volume ratios of DMA:PEG 400:waterat 2:2:1, 1:3:1, and 1:7:2. Using this approach, ten co-solvent systemsof various compositions and ratios were evaluated and optimized toachieve the maximal drug solubility for parenteral delivery. The MZsolubility in individual co-solvent formulations was determined by theHPLC assay. The optimal formulation was selected to be administeredintravenously (I.V.) as the most bioavailable formulation reference inthe preclinical bioavailability study of the selected lead oralmicroemulsion formulation.

Development of Microemulsion Formulations

Six self-emulsifying drug delivery systems (SEDDS) were prepared bymixing an oil, a low HLB surfactant, and a high HLB surfactant. Inparticular, 100 mg of MZ were dissolved in 5.5 ml of DMSO or DMA at 60°C. in a water bath. The solution was then mixed with Tween 80, Aralacel80, and various types of oils at different ratios.

A clear, transparent formulation was indicative of the formation of astable microemulsion. Phase diagrams were constructed with six differentratios of the ingredients for each system, by adding a small incrementof an aqueous phase (deionized water) to the SEDDS until the systembecame turbid, reflecting a phase separation, to define the boundary ofthe composition region for stable microemulsions.

Once the region of stable microemulsions was identified on the phasediagram, microemulsions were prepared by mixing appropriate quantitiesof the four ingredients (oil, dipolar aprotic solvent, surfactant, andwater) with a gentle hand shaking or stirring to ensure a throughmixing. MZ, either dissolved in DMA or DMSO, was incorporated by mixingwith the other ingredients.

Two optimal microemulsion formulations were selected for furtherassessment of in vitro drug release. One of the two optimal formulationswas evaluated for cell growth inhibition in cell culture models, and forin vivo preclinical bioavailability in rats. The lead microemulsionformulation will be suitable for future evaluation of anti-neoplasticefficacy in mice as well.

Determination of Drug Release

The drug release characteristics of the two selected SEDDS formulationwere comparatively evaluated with MZ powder and MZ solution in PEG 400.All four formulations were filled manually with a syringe into ahydrophilic airfill soft gelatin capsule (R. P. Scherer, Basking Ridge,N.J.) and the resulting hole was sealed thermally. USP XXII, DissolutionApparatus 2 (VanKel Industrial, Inc.) was employed to characterize therelease kinetics of MZ in vitro. Soft gelatin capsules containing theSEDDS, MZ solution, or MZ powder were individually placed in coppercoils to keep the capsules at the bottom of their respective dissolutionvessels. The vessels were filled with 500 mL of deionized watercontaining 0.5% Cremphor RH 40 (CMC=0.039%) at 37.degree. C. Cremphor RH40 was used to maintain the sink condition of the releasing medium. Therelease medium was constantly stirred at 50 rpm by a Teflon-coateddissolution paddle. Serial release samples were withdrawn and filteredthrough a 0.45 μm Millipore filter and analyzed by the HPLC assay.

Studies of Cell Growth Inhibition in NSCLC and Skin Cancer Cells UsingFormulation E4 and Placebo

Cells were plated in 96-well plates at 5×10⁶ cells/ml of Dulbecco'smodified Eagle's medium (DMEM) containing 100 U/ml penicillin, 100.mu.g/ml streptomycin, and 10% fetal serum albumin, and incubated at 37°C. in a 5% CO₂/95% air-humidified incubator for 24 hrs. Cells wereincubated for 48 hours after a medium change with fresh DMEM containingserial dilutions of E4 formulation or placebo. Cell survival wasmonitored with MTT assay. Anti-tumor effects of MZ were also evaluatedusing cell viability assays (trypan blue counting).

EXAMPLE 2

Preclinical Bioavailability of Microemulsion E4

Animals

Male Sprague-Dawley rats (Harlan Sprague Dawley, Inc., Indianapolis,Id.), weighing 300-400 g, were used after a 7-day acclimation period.Animals were housed under a 12 h light/dark cycle. A permanent catheterwas implanted in the right jugular vein of each rat on the day beforethe study, under the anesthesia with ketamine:acetopromazine:xylazine(50:3.3:3.3 mg/kg). Animals were fasted overnight, while water wasallowed ad libitum. On the morning of the experiment, rats were placedin individual metabolism cages.

Dosage Forms

The rats were randomized into three groups of three or four each. Ratsin Group 1 were injected intravenously through the jugular catheter witha single dose (3.25 mg/kg) of the P7 formulation in a cosolvent system.Group 2 received oral microemulsion formulation E4; at a single dose of5 mg/kg by oral gavage using an animal feeding needle (18×3″W/2¼ mmBall). Group 3 were dosed with MZ aqueous unformulated suspension at 50mg/kg similarly by oral gavage. The MZ suspension was prepared at 10mg/ml with a trace amount of glycerin as a wetting agent.

Blood Sampling

Serial blood samples approximately 0.6 ml each were collected inheparinized tube through the catheter at determined time intervals forup to 24 hours. The blood samples were immediately centrifuged at 14,000rpm for 3 min and the supernatant, plasma, was transferred into a 1.7-mlmicrocentrifuge tube and immediately stored at −80° C. until the HPLCassay.

HPLC Assay of Plasma Samples

MZ concentrations in plasma samples were determined by the HPLC methoddescribed in Example 1 with the following modifications. Briefly, 300 μlof plasma were mixed with 300 μl of acetonitrile to precipitateproteins. The acetonitrile contained 2 μg/mL quinidine (QD) as internalstandard. Prednisone was not used as IS for plasma samples, because itwas interfered by the broad solvent front from plasma blank. The mixtureof plasma and acetonitrile was vortexed and centrifuged, then 100 μl ofthe supernatant were withdrawn and injected directly onto the HPLCsystem. The mobile phase consisted of 35% (v/v) acetonitrile in 0.05 Maqueous solution of KH.sub.2PO.sub.4, pH 6.5. The flow rate was 1.2ml/min, and the effluent was monitored at 313 nm (0.01 AUFS).

Pharmacokinetic Analysis

Pharmacokinetic parameters were determined by fitting the plasmaconcentration-time data for each rat by a WinNonlin program. The maximum(peak) concentrations and the time to achieve maximum concentrations(peak time) in plasma were determined from the computer fitted plot.Areas under the concentration vs. time profiles from 0 to the last timepoint (720 min) of the measured concentration (AUC_(0→720)) wasdetermined by the linear trapezoidal rule, and the AUC from the time ofthe last measured concentration to infinity (AUC_(720→infin.)) wasdetermined by dividing the last determined concentration by the terminalphase elimination rate constant, which was initially estimated from theleast squares slope of the terminal linear segment of the plot. Theterminal phase half-life was calculated as 0.693 divided by the terminalphase elimination rate constant. Other non-compartmental pharmacokineticparameters were generated by area/moment analysis. Total plasmaclearance (CLT) was calculated from Dose/AUC, mean residence time (MRT)was calculated from AUMC/AUC, and steady-state volume of distribution(V.sub.ss) was calculated from [(Dose AUMC)/AUC²]. For oraladministration, bioavailability (F) was determined by the followingequation:F=[AUC _(oral) /AUC _(iv)]×[Dose_(iv)/Dose_(oral)]×100%Statistical Analysis

Differences between any two mean values were statistically evaluatedusing paired or unpaired Student's t-test subsequent to statisticalverification that the associated variances were homogeneous or equal.

EXAMPLE 3

HPLC Assay of MZ

The retention times of MZ and the IS (prednisone) were 9.3 min and 5.6min, respectively, under the HPLC eluting conditions aforementioned inExample 1. In particular, the stationary phase was a C₁₈ column, 300×3.9mm (Waters Corp.), the mobile phase was acetonitrile/0.05 M KH₂PO₄(40:60 V.V; pH=6.5), the flow rate was 1.2 ml/min, the detection was UVat 254 nm, for integration a Milton Ray CI-4100 integrator was used. Anauthentic HPLC chromatogram of MZ is shown in FIG. 1. Standard curves ofMZ were established in the concentration range of 0.05 μg/ml to 5.0μg/ml.

For the assay modified for plasma samples, the retention time for MZ andthe IS (QD) were 10 and 7 min, respectively. On each day of plasmasample assay, a calibration curve was constructed to calculate the MZconcentrations in plasma samples. The linearity of the curve wasestablished in a MZ concentration range of 0.05 to 2.5 μg/ml, withr²≧0.998. The calibration curve was plotted with peak area ratio ofMZ/QD versus the known MZ concentrations spiked in rat plasma blanks.

EXAMPLE 4

MZ Solubility in Various Solvent Media

The solubility of MZ in water and in the fourteen tested solvents at 25°C. are compiled in Table I.

TABLE I Solvent Medium Solubility (μg/mL) Enhancement Factor Water 0.0251 Methanol 55 2,200 PEG 400 528 21,120 Propylene glycol 196 7,840Triacetin 34.8 1,392 DMA 4,000 160,000 DMSO 5,000 200,000 Super refinedcottonseed oil 0.251 0 Olive oil 19.7 788 Super refined peanut oil 0.2510 Super refined soybean oil 5.8 232 Captex 200 13.3 532 Captex 355 1.248 Miglyol 812 40.6 1,624 Myvacet 9-45 39.3 1,572

MZ is poorly soluble in water with the solubility of 0.025 μg/mL. The MZsolubility in other solvent media, except super refined cottonseed oil,super refined peanut oil, and Captex 355, were all significantly higherthan its aqueous solubility, with enhancement factors ranging from 232to 200,000 in the reference to MZ aqueous solubility.

The MZ was favorably soluble in the six pharmaceutical solvents(methanol, PEG 400, propylene glycol, triacetin, DMA, and DMSO) withenhancement factors of 1,392 to 200,000. PEG 400, DMA, and DMSO yieldedthe highest three solubilities among the six tested solvents. PEG 400alone yielded a solubility of 0.528 mg/ml, still much lower than thetarget of 1.5 mg/mL.

DMA and DMSO freely dissolved MZ, but were not pharmaceuticallyacceptable to use as straight solvents. The LD.sub.50 of these solventsin rodents are 3.1 gm/kg. intravenously and 7.92 gm/kg orally. The oraland parenteral pharmaceutical products so far approved by FDA contain nomore than 40% of DMA or DMSO. Therefore, PEG 400, DMA, and DMSO wereselected to formulate MZ parenteral co-solvent systems with variouscompositions and ratios.

The MZ solubilities in the remaining five oils (olive oil, super refinedsoybean oil, Captex 200, Miglyol 812, and Myvacet 9-45) weresignificantly higher than its aqueous solubility, with enhancementfactors ranging from 232 to 1,624 (Table I). However, none of them alonecould reach the target concentration of 1.5 mg/ml. All the five oilscould be used to formulate MZ microemulsions.

EXAMPLE 5

Development of Parenteral Formulations: Co-Solvent Systems

Ten co-solvent systems, coded as P1-P10, containing PEG 400 and DMA orPEG 400 and DMSO with various ratios, were systematically evaluated fortheir capacity to dissolve MZ. The MZ solubility in individualco-solvent systems are tabulated in Table II.

TABLE II Formula Composition (Ratio) MZ Solubility Code Solvent ASolvent D Solvent E Water (mg/mL) Comment P1 2 2 0 0 0.574 Patentformula for Busulfan P2 2 2 0 1 0.459 Containing 40% of Solvent D P3 3 10 0 2.284 Optimal I.V. formulation with Solvent D P4 3 1 0 1 1.827Containing 20% of Solvent D P5 7 1 0 0 0.934 P6 7 1 0 2 0.747 Containing10% of Solvent D P7* 7 0 1 0 1.831 Optimal I.V. formulation with SolventE P8 7 0 1 2 1.440 Containing 10% of Solvent E P9 3 0 1 0 3.488 P10 3 01 1 2.790 Containing 20% of Solvent E *The selected parenteralformulatio, P7

The developed parenteral formulations yielded MZ solubility in the rangeof 0.46-3.49 mg/ml, 18,360-139,520 times higher than its aqueoussolubility. All the formulations except P2 yielded solubilities higherthan that in straight PEG 400, in the range of 1.09-6.61 times the MZsolubility in PEG 400. Six among the ten formulations, P3, P4, P7, P8,P9 and P10, yielded MZ solubility higher than 1.44 mg/ml, and wereconsidered promising and suitable for further in vitro and in vivoevaluations. Formulation P7 with MZ solubility of 1.83 mg/ml wasselected for future in vivo bioavailability study, based on its lowestcontent of DMSO and thus the maximal safety among the six promisingsystems.

EXAMPLE 6

Development of Microemulsion Formulations: Determination of Ability toIncorporate Water Formulations

Thirty-six microemulsions (A1-A6, B1-B-6, C1-C-6, D1-D6, E1-E6, andF1-F6) were formulated and their capacities to incorporate water and todissolve MZ were evaluated. These systems contained an oil (superrefined soybean oil, Captex 200, Miglyol 812, or Myvacet 9-45), asolvent (DMA or DMSO), a blend of a high HLB Surfactant Tween 80 and alow HLB Surfactant Arelacel 80 (in a ratio of 1:1 or 2:1), and anaqueous phase. Their compositions are given in Tables III-A-III-F. Inthese Tables, the following codes are used: J=super refined soybean oil;K=Captex 200; M=Miglyol 812; N=Myvacet 9-45; D=DMA; E=DMSO; O=Tween 80;P=Arelacel 80.

Among the formulated microemulsions, twenty-two formulations (A1-A3,B1-B3, C₁-C₄, D1-D4, E1-E4, and F1-F4) yielded MZ concentrations in therange of 1.5-2.64 mg/ml, meeting the target concentration of 1.5 mg/ml.When the maximum water that could be incorporated was taken intoconsideration, sixteen of the twenty-two microemulsion formulations, A3,B3, C1-C4, D3-D4, E1-E4, and F1-F4, that incorporated 10% (by weight) orhigher of aqueous medium, were considered more stable than the restformulations. Twelve microemulsions, C₁-C₄, E1-E4 and F1-F4 were themost promising formulations. Microemulsion E4 was selected for in vitrodrug release and future in vivo studies of bioavailability andanti-neoplastic activity. In release study, formulation A4 was alsocomparatively evaluated. A representative pseudo-ternary phase diagramindicating the compositions of microemulsions E1-E6 is shown in FIG. 2.

TABLE III-A Composition (% by Weight) MZ Formulation Oil SolventSurfactant H₂O Conc. Code J K M N D E O P Incorporation HLB (mg/mL) A1 —50.6 — — 24 — 12.7 12.7 4 9.65 2.19 A2 — 44.9 — — 21.3 — 16.9 16.9 4.59.65 1.93 A3 — 40.4 — — 19.2 — 20.2 20.2 11 9.65 1.89 A4 — 28.9 — — 13.7— 28.8 28.8 13 9.65 1.13 A5 — 25.3 — — 11.9 — 31.4 31.4 13 9.65 0.98 A6— 13.4 — — 6.4 — 40.1 40.1 14 9.65 0.5

TABLE III-B Composition (% by Weight) MZ Formulation Oil SolventSurfactant H₂O Conc. Code J K M N D E O P Incorporation HLB (mg/mL) B1 —47.9 — — 22.8 — 16 8 5.3 11.4 2.25 B2 — 41.9 — — 19.9 — 20.9 10.6 6.711.4 2 B3 — 36.2 — — 17.2 — 24.1 12.1 10.3 11.4 1.8 B4 — 24.8 — — 11.8 —33.1 16.5 13.9 11.4 1.29 B5 — 24.4 — — 11 — 32.5 16.3 15.9 11.4 1.3 B6 —11.1 — — 5 — 46.5 23.3 14.1 11.4 0.58

TABLE III-C Composition (% by Weight) MZ Formulation Oil SolventSurfactant H₂O Conc. Code J K M N D E O P Incorporation HLB (mg/mL) C1 —50.1 — — — 12.6 12.6 12.6 12.1 9.65 2.64 C2 — 42.6 — — — 10.7 16.1 16.114.5 9.65 2.31 C3 — 38.4 — — — 9.6 19.2 19.2 13.6 9.65 2.06 C4 — 34.7 —— — 8.7 21.7 21.7 13.2 9.65 1.85 C5 — 26.5 — — — 6.7 26.7 26.7 13.4 9.651.42 C6 — 17.2 — — — 4.3 32.5 32.5 13.5 9.65 0.92

TABLE III-D Composition (% by Weight) MZ Formulation Oil SolventSurfactant H₂O Conc. Code J K M N D E O P Incorporation HLB (mg/mL) D1 —— — 50.6 24 — 12.7 12.7 0 9.65 2.25 D2 — — — 44.9 21.3 — 16.9 16.9 09.65 2 D3 — — — 36.4 17.3 — 18.2 18.2 9.9 9.65 1.8 D4 — — — 33.1 15.6 —20.7 20.7 9.9 9.65 1.64 D5 — — — 25.5 12.1 — 25.5 25.5 11.4 9.65 1.29 D6— — — 16.9 8.1 — 31.4 31.4 12.2 9.65 0.87

TABLE III-E Composition (% by Weight) MZ Formulation Oil SolventSurfactant H₂O Conc. Code J K M N D E O P Incorporation HLB (mg/mL) E156.5 — — — — 14.1 14.1 14.1 1.2 9.65 2.14 E2 44.2 — — — — 11.1 16.6 16.611.5 9.65 1.88 E3 40 — — — — 10 20 20 10 9.65 1.87 E4 35.7 — — — — 8.922.3 22.3 10.8 9.65 1.8 E5 27.4 — — — — 6.9 27.6 27.6 10.5 9.65 1.15 E619 — — — — 4.7 33.2 33.2 9.9 9.65 0.79 *The selected Formulation E4comprised of Oil J:Solvent E:Surfactant O:Surfactant P = 2 g:0.5 mL:1.25g:1.25 g

TABLE III-F Composition (% by Weight) MZ Formulation Oil SolventSurfactant H₂O Conc. Code J K M N D E O P Incorporation HLB (mg/mL) F1 —— 50.4 — — 12.6 12.7 12.7 11.6 9.65 2.14 F2 — — 42.4 — — 10.6 15.9 15.915.2 9.65 1.88 F3 — — 39.2 — — 9.8 19.6 19.6 11.8 9.65 1.67 F4 — — 34.7— — 8.7 21.8 21.8 13 9.65 1.5 F5 — — 27.2 — — 6.8 27.3 27.3 11.4 9.651.15 F6 — — 18.4 — — 4.6 32.8 32.8 11.4 9.65 0.78MZ Release from Microemulsions A4 and E4

The comparative in vitro release kinetics of MZ from drug powder,solution in PEG 400, microemulsion A4 (containing DMA), andmicroemulsion E4 (the selected oral formulation, containing DMSO),respectively, were evaluated and summarized in Table IV. The cumulativerelease profiles are presented in FIG. 3. MZ was readily released fromthe microemulsions A4 and E4, with 78.7% and 74.2%, respectively,dissolved in 30 minutes, greatly enhanced as compared with that ofunformulated drug powder, 0.3% in 60 minutes. The MZ solution in PEG 400was precipitated when in contact with the release medium after thecapsule shell was disintegrated. The MZ dissolved slowly thereafter, andreached 17.1% dissolved in 60 minutes (Table IV).

TABLE IV Cumulative MZ Released (% Dose) Time MZ Mean MZ MeanFormulation Mean Formulation Mean (min) powder (SD) in PEG (SD) A4* (SD)E4** (SD) 10 0.01 0.86 3.9 0.48 0.012 1.1 60 26.7 0.083 0.04 2.7 1.5559.8 41.23 43.9 23.69 (0.04) (1.00) (32.33) (21.87) 20 0.035 9.0 76.872.2 0.22 9.7 67 61.3 0.18 0.15 9.9 9.53 78.8 74.20 71.1 68.20 (0.10)(0.47) (6.32) (6.00) 30 0.14 20.9 74.9 67.6 0.25 10.2 83.4 74.3 0.280.22 — 15.56 77.7 78.67 80.8 74.23 (0.07) (7.57) (4.33) (6.60) 40 0.2224.3 82.3 71.0 0.23 12.2 78 72.5 0.41 0.29 12.1 16.20 80.4 80.23 69.370.93 (0.11) (7.02) (2.16) (1.60) 50 0.2 22.7 — 85.1 0.31 10.4 80 73.90.35 0.29 8.3 13.80 89.7 84.85 81.3 80.10 (0.08) (7.78) (6.86) (5.70) 600.27 27.5 98.1 75.4 0.27 11.3 77 79.4 0.32 0.29 12.5 17.10 86.4 87.1778.0 77.60 (0.03) (9.03) (10.57) (2.03) *A4 Formulation - Oil K:SolventD:Surfactant O:Surfactant P = 2 g:1 mL:2 g:2 g **E4 Formulation - OilJ:Solvent E:Surfactant O:Surfactant P = 2 g:0.5 mL:1.25 g:1.25 gPreclinical Bioavailability Evaluation of E4

Eleven rats were used for the preclinical bioavailability evaluations ofthe lead oral microemulsion formulation in three independent runs. Therats were randomly grouped into three treatment groups: (a) I.V. dose of3.25 mg/kg, n=3, (b) oral E4 microemulsion dose of 4.89 mg/kg, n=4, and(c) oral suspension dose of 50 mg/kg, n=4 (Table V). Table VI shows thepharmacokinetic parameters of MZ from P7, E4 and oral suspensionformulations in rats.

TABLE V Rat Weight Dose Cmax AUC₀₋₇₂₀ Tmax t_(1/2) Bioavail. No RouteDosage Form (g) (mg/kg) (ng/mL) (ng min/mL) (min) (min) (%) M IVCo-solvent 300 3.25 10849 733042 — 104 N IV Co-solvent 320 3.25 13747876594 —  77 O IV Co-solvent 320 3.25 7699 914010 — 120 Mean ± SD 313 ±12 3.25 10765 ± 3025 841635 ± 95766 100 ± 22 A Oral Microemulsion 3254.89 867 526350 240 — 41.6 E Oral Microemulsion 365 4.89 794 327660 240127 25.9 F Oral Microemulsion 375 4.89 697 337680 240 231 26.7 G OralMicroemulsion 360 4.89 1064 354270 120 129 28.0 Mean ± SD 356 ± 22 4.89 856 ± 155 386490 ± 93883 210 ± 60 162 ± 59 30.6 ± 7.4  C OralSuspension 303 50 154 42165 300 115 0.33 H Oral Suspension 345 50 13838970 360 102 0.30 I Oral Suspension 370 50 168 64140 360 191 0.50 JOral Suspension 380 50 63 14940 240 — 0.12 Mean ± SD 350 ± 34 50 131 ±47 40054 ± 20137 315 ± 57 136 ± 48 0.31 ± 0.16

TABLE VI Plasma Concentration (ng/ml) Time IV E4 Suspension (min) M N OA E F G C H I J 0 10849 13747 7700 0 0 0 0 0 0 0 0 5 13365 7191 7 916215 7553 12633 6272 30 4599 6527 4601 646 502 216 534 60 2539 2846 3430562 490 310 854 22 26 35 75 14 90 1848 2371 2664 120 1654 1924 2235 842555 533 1064 26 13 58 52 180 1078 1362 1587 71 240 781 842 1274 867 794697 840 64 90 63 300 469 371 789 154 360 187 779 567 558 449 138 168 10480 787 374 550 232 43 60 116 720 556 83 164 12 12 46

The plasma-time profiles of MZ from I.V., E4 and unformulatedsuspensions were constructed. (FIGS. 4-6). The semi-log plot of theprofiles of MZ after I.V. administration to individual rats (FIG. 7)were all bi-phasic with a rapid decline in mean concentrations from 7699ng/ml to 2662 ng/ml within 90 min. The profile fitted a two-compartmentmodel, indicating that the distribution of MZ in rats had a distincttissue peripheral compartment. The distribution process appeared to befaster than the absorption process when MZ was given orally. Theprofiles of MZ from administrations of oral E4 and suspension did notexhibit any bi-phasic decline and fitted well into the typical1-compartment model with an absorption process of first-order kinetics.The drug concentrations from suspension dosing (10-168 ng/ml) weresubstantial lower than those from E4 dosing (83-1064 ng/ml), even thesuspension dose administered was 10 times higher.

The plasma profiles were analyzed by non-compartmental model withWinNonlin program to derive pharmacokinetic parameters for comparison.The MZ peak concentration from E4 was 856±155 ng/ml, 6.5 folds of thatfrom suspension, 131±47 ng/ml. (Table V). The peak time from both dosingwere comparable, 210±60 and 315±57 min, for E4 and suspension,respectively. The absorption half-lives were similar, 100±57 and 184±63min, for E4 and suspension, respectively. The biological half-life wasnot statistically different from the different formulations (I.V.solution, E4 microemulsion and suspension) nor by the various routes ofadministration (I.V. and oral.), ranging from 100-162 min.

The systemic exposure of MZ after the different dosage forms weremeasured by AUC₀₋₇₂₀. When the values were normalized by the respectivedoses given, the absolute bioavailability of E4 and suspension inreference to I.V. dosing were 30.6% and 0.31%, respectively (Table V).The relative bioavailability of E4 in reference to the unformulatedsuspension was 98.7. It means that by formulating MZ into themicroemulsion formulation, the bioequivalent dose of E4 was about 1/100of that of the unformulated suspension.

Cell Growth Inhibition in NSCLC and Skin Cancer Cell Lines

The E4 formulation and placebo were used in studies of cell growthinhibition in skin cancer cell lines (SRB1, A375 and 2237) and non-smallcell lung cancer (NSCLC) cell lines. The dose-response curves for cellgrowth inhibition were established for the range of 1.7-170 ng/ml forskin cancer cells (FIG. 8) and 2.5-250 ng/ml for NSCLC (FIG. 9). TheIC.sub.50 of MZ in skin cancer cells was 91, 129, and 171 ng/ml forSRB1, A375, and 2237, respectively (FIG. 8). The IC.sub.50 of MZ fromtwo microemulsion formulations in NSCLC cells was 8 and 10 ng/ml,respectively, which represented 8-9 fold enhancements, as compared withthose of DMSO-dissolved MZ controls (FIG. 9). Thus, this study ofenhanced anti-neoplastic activity in cultured cells provides evidence ofthe effective delivery of MZ by the E4 formulation. Thus, the developedmicroemulsion formulations exhibited favorable characteristics in drugrelease, preclinical efficacy in cultures, and preclinicalpharmacokinetics in rats.

EXAMPLE 7

Optimized SNEDDS. SEDDS and Microemulsions for Parenteral Administrationwith Increased Bioavailability

Pre-Formulation Studies

An HPLC assay for mebendazole was validated within the linear range of0.02-10 μg/ml for both aqueous buffer and plasma samples. Mebendazolewas identified to be a weak base, with log P of 2.51, belonging toBiopharmaceutical Classification Scheme II of drugs. Thus, formebendazole, permeability is not a major factor limiting drugabsorption; rather dissolution was identified to be the rate-limitingstep for the systemic absorption of the drug. The pH-stability studyindicated that the drug was sufficiently stable at room temperature inthe pH range of 1-7, for a period of 33 days. Thus, no pH-relatedstability issues were identified for mebendazole that could limit drugabsorption. The effective pH for the formulation of mebendazole wasidentified to lie in the range of 3-7.

Solubility of Mebendazole in Various Oils

The solubility of Mbz was determined in different vehicles and phasediagrams (FIGS. 10A-10B) constructed to define the microemulsion regionsfor selections of the lead SEDDS and SNEDDS. The solubilities ofmebendazole in various natural and other oils are listed in Table VIIand in various surfactants and cosurfactants in Table VIII. Oils andsurfactants/cosurfactants selected for SNEDD and SEDD development aremarked (*). Each value in Tables VII and VIII represents the mean±SD ofthree independent determinations.

TABLE VII Solubility Oil Oil type (μg/ml) Super refined: Cotton seed oilNatural oil 3.9 ± 0.1 Sesame seed oil Natural oil (Sesamum Indicum) 1.7± 1.0 Soybean oil Natural oil (Glycine soja)  5.8 ± 1.3* Corn oilNatural oil 2.8 ± 0.5 Peanut oil Natural oil 2.4 ± 0.9 Shark liver oilNatural oil 6.1 ± 0.6 Captex 200 C₈/C₁₀ diesters of PG from coconut oil 9.0 ± 0.9* Myglyol C₈/C₁₀ triglycerides from coconut oil 15.2 ± 0.8*Myvacet 9-45K Distilled acetylated monoglyceride 61.3 ± 1.2* Olive oilNatural oil 2.7 ± 0.6 Neobee M-5 C₈/C₁₀ caprylic/capric triglyceride 5.8± 1.4

TABLE VIII Surfactants/Cosurfactants Solubility (HLB) S/CoS type (μg/ml)Labrafac CC (10.0) Medium chain triglyceride EP 32.31 ± 5.0  (C₈-C₁₀fatty acid) Labrasol (14.0) Caprylocaproyl macrogol-8 605.53 ± 52.6*glycerides Labrafil M 1944 CS (3-4) Oleoyl macrogol-6 glycerides 88.36 ±32.4 Labrafil M 2125 CS (3-4) Linoleoyl macrogol-6 glycerides 178.81 ±22.6  Aracel 80/Crill 4NF (4.3 Sorbitan Oleate 101.29 ± 2.0*  Capmul MCM(5.5-6.0) C₈/C₁₀ mono-/diglyceride from 494.52 ± 60.4* coconut oilCremophor RH 40 Polyoxyl 40 hydrogenated 91.11 ± 31.6 (14.0-16.0) castoroil Cremophor EL (13.5) Polyoxyethylenglycerol- 346.51 ± 61.2*triricinoleat Crillet 1-HP (16.7) Polyoxyethylene (20) sorbitan 31.0 ±5.1 monolaurate (Polysorbate 20) Crillet 4-HP (15.0) Polyoxyethylene(80) sorbitan 370.18 ± 49.2* monolaurate (Polysorbate 80) Centrophase152 Soy lecithin 95.44 ± 4.6  (Mixed phospholipids) Triacetin1,2,3-propanetriol/glyceryl- 54.44 ± 5.12 triacetate/triacetyl glycerolTranscutol P Diethylene glycol monoethyl  299.1 ± 9.57* ether

FIG. 10A is a pseudo-ternary phase diagram for SNEDDs using asurfactant/cosurfactant combination. Points A1-A6, represent thedifferent weight compositions of surfactant, cosurfactant, oil and waterincorporated in the formulation to delineate the regions ofmicroemulsion existence. The line joining the points A1-A6 is theboundary line. The area above the boundary line represents the region ofphase separation, and the area below the line is the region ofmicroemulsion existence. Various compositions were evaluated within thecordoned area of microemulsion existence to determine the region ofefficient emulsification. Compositions marked under “region of interest”were those having a high efficiency of emulsification and can be dilutedto maximum amounts of water without drug precipitation or phaseseparation. Compositions marked under the “region of interest” were thenoptimized to find the formulate Type IIIB system/SNEDDS with a dropletsize of <50 nm and a targeted drug solubility of 1.96 mg/ml.

FIG. 10B is a pseudo-ternary phase diagram for SNEDDs using a high/lowHLB surfactants combination. Points B1-B5, represent the differentweight compositions of surfactant, cosurfactant, oil and waterincorporated in the formulation to delineate the regions ofmicroemulsion existence. The line joining the points B1-B5 is theboundary line. The area above the boundary line represents the region ofphase separation, and the area below the line is the region ofmicroemulsion existence. Various compositions were evaluated within thecordoned area of microemulsion existence to determine the region ofefficient emulsification. Compositions marked under “region of interest”were those having a high efficiency of emulsification and can be dilutedto maximum amounts of water without drug precipitation or phaseseparation. Compositions marked under the “region of interest” were thenoptimized to find the formulate Type II system/SEDDS with a droplet sizeof <200 nm and a targeted drug solubility of 1.96 mg/ml.

Table IX lists formulations or compositions for SNEEDs, SEDDs andparenteral microemulsions (PMs) utilizing the selected oils, surfactantsand cosurfactants.

TABLE IX SNEDDS SEDDS PM-1 PM-2 Size (nm) 35 143 37 478 Drug conc.(mg/ml) 1.96 1.96 0.95 0.95 Compositions (% w/w) Captex 200 (oil) 9.0 —4.5 18.0 Myglyol (MCT) (oil) — 42.0 — — Tween 80 (surfactant) 40.5 2720.25 13.5 Capmul (cosurfactant) — 21 — — Transcutol (cosurfactant) 40.5— 20.25 13.5 DMSO (vehicle) 10.0 10.0 5.0 5.0 Water — — 50.0 50.0

Captex 200/Tween 80/Transcutol and Myglyol/Tween 80/Capmul MCM systemsled to the formation of efficient SNEDDS and SEDDS, respectively, thatresulted in a high efficiency of emulsification and could be dilutedwith water to the maximum extents without phase separations. No changein the droplet size was observed at 10% w/w of mebendazole in theformulated SNEDDS and SEDDS, with mean droplet diameters of 34.8±2.1 nmand 143.0±2.0 nm and, respectively, which were similar to the dropletsizes of the placebo formulations.

Mebendazole Release Profiles

The rates of drug release was rapid, 0.053±0.002% min⁻¹ and 0.078±0.004%min⁻¹ for the SNEDDS and SEDDS, respectively, which were significantlygreater than that of the unformulated suspension, 0.004±0.001% min⁻¹.Mebendazole was readily released from SNEDDs and SEDDs with 92.4 and89.4% of drug released in 30 minutes, respectively, compared to 79.6 and0.30% of drug release in 60 minutes from the co-solvent and unformulatedsuspension, respectively (FIG. 11A). Each point represents the mean±SDof three independent determinations. The release profiles of the SNEDDSand SEDDS were similar to each other.

The extent of drug release from unformulated suspension in release mediaand unformulated drug in release media with placebo SNEDDS was 0.30% and1.10%, respectively, at the end of 60 minutes (FIG. 11B). Each pointrepresents the mean±SD of three independent determinations. The presenceof placebo SNEDDS in the dissolution medium did not significantlyenhance the rate or the extent of dissolution mebendazole to the extentsof increase that were obtained from the SNEDDS and SEDDS. Therefore, theeffects of wetting agent, glycerin, and the placebo SNEDDS cannot beaccounted for the extents of increases of mebendazole dissolution fromthe SEDDS and SNEDDS. Hence, the enhanced dissolution observed with theSEDDS and SNEDDS is a formulation effect and not a solvent effect.

The extents of free drug released after 60 minutes were 77.9±3.6% and74.7±9.4% from SNEDDS and SEDDS, respectively. The free drug moleculesconstitute 79.4% and 75.2% of the total released mebendazole, 98.1 and99.4%, from SNEDDS and SEDDS, respectively (data not shown). Therefore,the majority of the drug molecules that were released from the SNEDDSand SEDDS were present in the free form.

Table X lists the f₂ similarity values, calculated using the equationsdescribed below, for the SNEDDs, SEDDs and cosolvent formulations. An f₂value between 50-100 suggests that the two dissolution profiles aresimilar. The asterisk* depicts significance between groups comparedafter analysis by ANOVA using Tukey's post-hoc test, at a threshold ofsignificance at p<0.05.

TABLE X f₂ Similarity Values SNEDDS/ SEDDS/ SNEDDS/ Extent of ReleaseCosolvent Cosolvent SEDDS 0-20 minutes 71.0 77.1 92.6 20-60 minutes56.0* 54.5* 97.3 Rate of Release 75.6 78.5 94.9 0-20 minutes

Both the SNEDDS and SEDDS were physically stable in the GI fluidswithout any changes in their droplet sizes. Further, the drug waschemically stable in the GI fluids (SGF, SIF) for 4 hours. Increaseddilutions did not affect the physical stability of the formulation orthe chemical stability of the drug. SNEDDS and SEDDS formulations werealso chemically stable with drug contents of 97.8±0.6% and 98.2±0.4%,respectively, and over a period of one and one-half year at roomtemperature.

The lead parenteral cosolvent formulation as a reference for oralbioavailability studies was stable for over a two-week period havingdrug concentrations of 2.0±0.1 (n=6) mg/ml and incorporated the leastamount of DMSO (10% w/w). The absolute oral bioavailabilities of theSNEDDS, SEDDS and the unformulated suspension administered orally were70.7%, 37.4% and 0.3%, respectively, in reference to the I.V. cosolventformulation. The relative bioavailabilities of SNEDDS and SEDDS, inreference to the unformulated suspension were 228% and 120%,respectively, while the SNEDDS formulation had approximately two foldhigher oral bioavailability compared to SEDDS. The SNEDDS and SEDDSformulations were designed rationally to identify the relativecontributions of the effects of particle size and lipid digestion on thebioavailability enhancement processes. The particle size was the drivingfactor for enhanced absorption from SNEDDS of 35 nm, while lipiddigestion played an important role in enhancing bioavailability for theSEDDS.

Table XI provides a summary of vital pharmacokinetic parameters ofmebendazole in rats after oral administration of SNEDDS, SEDDS,unformulated suspensions and parenteral cosolvent formulations.

TABLE XI Formulations Pharmacokinetic SNEDDS SEDDS UnformulatedCosolvent Parameters (35 nm) (143 nm) Suspension Formulation No. ofsubjects n = 4 n = 5 n = 4 n = 6 Route of p.o p.o p.o i.v. AdminstrationDose (mg/kg) 5.0 5.0 50.0 3.25 C_(max) or C_(o(i.v.)) 1.89 ± 0.37** 0.58 ± 0.06* 0.13 ± 0.05 11.30 ± 2.65  (μg/ml) AUC (min * μg/ml) 987.76± 112.16** 522.40 ± 14.40* 40.05 ± 20.14 908.63 ± 148.23 t_(max) (min)67.0 ± 23.6** 129.4 ± 39.4* 315.0 ± 57.0  — Absolute 70.7** 3.74* 0.30100 Bioavailability (%) Relative 228* 120* 100 — Bioavailability (toUnformulated Suspension)Mean Plasma-Concentration Time Profiles

Groups of Sprague-Dawley rats were administered mebendazole eitherparenterally via an i.v bolus or orally via oral gavage and the meanplasma-concentration time profile was measured over a period of about 12hours. SD rats were administered 1) 3.25 mg/kg (n=6) of a parenteralcosolvent formulation intravenously (FIG. 12A); 2) 5.0 mg/kg (n=4) ofSNEDDS orally (FIG. 12B); 3) 5.0 mg/kg (n=5) of SEDDS orally (FIG. 12C);4) 50.0 mg/kg (n=4) of an unformulated suspension orally (FIG. 12D); 5)1.60 mg/kg (n=3) of each of parenteral microemulsions PM1 and PM2intravenously (FIGS. 12E-12F). Note in FIG. 12D that the standarddeviations of the plasma-drug concentrations are very high because ofslow and erratic absorption of mebendazole from the unformulatedsuspension. The hemolytic potential, H10%, of the formulation isinterpolated from FIG. 12G to be 0.2. Thus, because of the substantiallylow surfactant/cosurfactant content, i.e., about 6% to about 48%,preferably about 27% to about 42%, of SNEDDS and microemulsions PM1 andPM2, these formulations are hemolytically safe for parenteraladministration. With increased volumes of the formulation used, thehemolysis increased with 90% of the cells hemolyzed at a formulation toblood ratio of 1.5.

Table XII provides a summary of pharmacokinetic parameters fromintravenous cosolvent and microemulsion formulations in rats. All valuesare shown as mean±S.D. Differences between any two means werestatistically evaluated using ANOVA, with Tukey's post-hoc analysis, ata threshold of significance at p<0.05. The *p<0.05 for comparisonbetween cosolvent and microemulsion formulations.

TABLE XII I.V. Pharmaco- Formulations kinetic MicroemulsionMicroemulsion Parameters Cosolvent (PM1-37 nm) (PM2-478 nm) No. of SDrats n = 6 n = 3 n = 3 Dose (mg/kg) 3.25 1.6 1.6 AUC (μg * 908.6 ± 148.2159.7 ± 11.1  170.2 ± 2.1  min/ml) AUC/Dose 279.6 ± 45.6* 99.8 ± 6.9 106.4 ± 1.3  C_(max) (μg/ml) 11.3 ± 2.7  2.0 ± 0.1 1.8 ± 0.1C_(max)/Dose  3.5 ± 0.8* 1.3 ± 0.1 1.1 ± 0.1 t_(1/2,) α (min) 17.0 ±3.6  17.9 ± 4.9  24.0 ± 3.9  t_(1/2,) β (min) 173.4 ± 100.3 114.1 ±27.0  145.7 ± 21.4  Clearance  3.7 ± 0.6* 10.1 ± 0.7  9.4 ± 0.1 (ml/min)Vss (ml)  692.2 ± 265.6* 1353.6 ± 152.3  1584.7 ± 212.3  V₁ (ml) 303.4 ±69.1* 814.4 ± 40.4  895.0 ± 52.6  V₂ (ml) 388.8 ± 249.3 539.3 ± 189.3689.7 ± 172.7 α (min⁻¹) 0.043 ± 0.009 0.041 ± 0.010 0.030 ± 0.005 β(min⁻¹) 0.005 ± 0.003 0.006 ± 0.001 0.005 ± 0.001 k₁₀ (min⁻¹) 0.013 ±0.004 0.012 ± 0.001 0.011 ± 0.000 k₁₂ (min⁻¹) 0.018 ± 0.005 0.013 ±0.002 0.022 ± 0.010 k₂₁ (min⁻¹) 0.017 ± 0.006 0.022 ± 0.010 0.013 ±0.003Plasma Pharmacokinetics of Cosolvent and Microemulsions PM1 and PM2 inMice

Plasma pharmacokinetics for Mbz from cosolvent and microemulsions (PM1and PM2) in mice have been studied. A naive averaged data approach wheremean concentration-time profiles were generated by calculating the meanconcentration at each time point was employed for each formulation. Themean plasma pharmacokinetic parameters were derived from the meanconcentration-time profile for each formulation by WinNonlin (TableXIII). Therefore, the values of pharmacokinetic parameters werepresented as mean values derived from mean concentration-time profiles.No standard deviations were presented and statistical analysis was notperformed. Bioavailability, BA, is the ratio of the AUC/dose of PM1 orPM2 to that of cosolvent.

For all of these three formulations, the Mbz plasma concentrationdeclined rapidly after injection and was too low to be detected after 6hr (FIG. 13). The plasma concentration-time profiles of Mbz from thesethree formulations following i.v. injection in mice were best fitted ina two-compartment model. Curves for three concentration-time profilesdisplayed short distribution a phase (t_(1/2), α<0.1 hr), whichindicated a rapid distribution phase (Table XIII). The Mbzpharmacokinetic parameters such as AUC/dose, t_(1/2)α, t_(1/2), β, andCL were comparable among groups of cosolvent, PM1 and PM2.

However, the C_(max/dose) of cosolvent was 1.45 (mg/L)/(mg/kg), onlyabout half of those of PM1 and PM2, 3.49 and 3.24 (mg/L)/(mg/kg),respectively. In addition, k₁₀ for cosolvent was 1.27 hr⁻¹, which wasabout half of those for PM1 and PM2, 2.87 and 2.25 hr⁻¹, respectively.k₂, for cosolvent was 3.57 hr⁻¹, which was 2 times higher than those forPM1 and PM2, 1.62 and 1.76 hr⁻¹, respectively. The relativebioavailability was 1.07 and 1.26 for PM1 and PM2, respectively. Incontrast to these differences between Mbz cosolvent and microemulsions(PM1 and PM2), all plasma pharmacokinetic parameters were comparablebetween PM1 and PM2.

TABLE XIII Pharmacokinetic Parameters Units Cosolvent PM1 PM2 Dose mg/kg6.5 3.25 2.5 C_(max)/Dose (mg/L)/(mg/kg) 1.45 3.49 3.24 AUC/Dose (hr *mg/L)/(mg/kg) 1.14 1.22 1.44 t_(1/2,) _(—) hr 0.078 0.068 0.095 t_(1/2,)_(—) hr 1.34 1.52 1.27 α 1/hr 8.84 10.01 7.28 β 1/hr 0.51 0.46 0.54 CLL/hr 0.029 0.027 0.023 V_(ss) L 0.051 0.044 0.032 V₁ L 0.023 0.009 0.010V₂ L 0.028 0.035 0.022 k₁₀ 1/hr 1.27 2.87 2.25 k₁₂ 1/hr 4.52 6.17 3.81k₂₁ 1/hr 3.57 1.62 1.76 Relative BA 1.07 1.26Biodistributions of Mbz from Cosolvent and Microemulsions (PM1 and PM2)in Mice

Different tissue distribution patterns among Mbz from cosolvent, PM1 andPM2 were observed which were not anticipated based on their plasmapharmacokinetic profiles (FIGS. 14A-14C). Mbz peak concentrations indifferent organs were reached by 5 min after injection for allformulations. For cosolvent, the top two highest peak concentrationswere 1.82 (μg/g)/(mg/kg) in liver and 1.50 (μg/g)/(mg/kg) in kidneys, incontrast to 4.28 (μg/g)/(mg/kg) in lung, 1.94 (μg/g)/(mg/kg) in liver,and 1.82 (μg/g)/(mg/kg) in kidneys for PM1, and 7.46 (μg/g)/(mg/kg) inlung, 2.40 (μg/g)/(mg/kg) in kidneys, 2.24 (μg/g)/(mg/kg) in liver forPM2. Table XIV presents the biodistributions for cosolvent formulationsand parenteral microemulsions PM1 with a droplet size of 37 nm and PM2with a droplet size of 478 nm in mice (n=4-5).

Mbz in cosolvent yielded the highest exposure in liver with an AUC of3.37 (hr*μg/g)/(mg/kg), followed by kidneys of 2.70 (hr*μg/g)/(mg/kg).However, for PM1, the AUC in lung [12.38 (hr*μg/g)/(mg/kg)] was thehighest, followed by those in liver [2.69 (hr*μg/g)/(mg/kg)] and kidneys[2.19 (hr*μg/g)/(mg/kg)]. For PM2, the AUC in lung [10.82(hr*μg/g)/(mg/kg)] was the highest too, followed by those in liver [2.95(hr*μg/g)/(mg/kg)] and kidneys [2.78 (hr*μg/g)/(mg/kg)]. Comparing themicroemulsion group (PM1 and PM2) with Mbz cosolvent, the AUCs in lungfrom PM1 and PM2 were 6-7 times, and the AUC in brain were 50%-60% ofthose from MBz cosolvent, respectively. The AUCs in the rest organs werecomparable between Mbz microemulsions and cosolvent. The AUCs in all sixorgans were comparable between PM1 and PM2.

For cosolvent, the elimination half-lives of Mbz were similar in heart,lung, liver, spleen, and kidneys, 1.96, 1.60, 1.63, 1.79, and 1.83 hr,respectively. The elimination half-life in lung (4.54 hr) wassubstantially prolonged for PM1, which was about 3 times of that fromcosolvent. Half-lives of Mbz from PM1 in other organs except brain were1.38, 1.55, 1.40, and 1.44 hr in heart, liver, spleen and kidneys,respectively, similar to those from cosolvent. Half-lives of Mbz fromPM2 in all organs except brain were 1.18, 1.27, 1.15, 1.03 and 1.08 hrin heart, liver, spleen and kidneys, respectively, similar to those fromcosolvent and PM1. Half-lives of Mbz in brain from PM1 and PM2 werecomparable, 1.05 and 0.93 hr, respectively, but much shorter than thatfrom cosolvent (2.85 hr).

Comparing Mbz concentrations from cosolvent, PM1 and PM2 at 5 min, asignificantly higher Mbz concentration at 5 min in spleen from PM2 thanthose from cosolvent and PM1 was found. In addition, the Mbzconcentrations in lung and brain at 5 min from PM1 and PM2 weresignificantly higher than those from cosolvent too. The peak Mbzconcentrations in liver and kidney were comparable among these threeformulations (FIGS. 15A-15C). At 2 hr, PM1 and PM2 still possessedsignificantly higher Mbz concentrations in lung than cosolvent. At 4 hr,the Mbz concentrations in lung from PM1 and PM1 were significantlyhigher than that from cosolvent. In contrast, the Mbz concentrations inspleen, heart and brain were significantly lower than that fromcosolvent.

The observation of substantial distributions of Mbz in lung from PM1 andPM2 was not anticipated. The retention of Mbz from PM1 in lung wasprolonged from that of cosolvent. Nevertheless, the retained Mbz fromPM2 was eliminated faster, resulting in a similar half-life to that ofcosolvent. The significantly greater AUC and prolonged half-life of Mbzin lung from PM1 may offer potential merits of Mbz delivery fortreatments of lung cancer and pulmonary infections.

TABLE XIV PARAMETERS Heart Lung liver spleen kidneys Brain CosolventC_(max) 0.90 0.85 1.82 0.79 1.50 0.90 (μg/g)/(mg/kg) AUC_(0-4 hr) 1.791.76 3.37 1.57 2.70 2.49 (hr * μg/g)/(mg/kg) t_(1/2) (hr) 1.96 1.60 1.631.79 1.83 2.85 PM1 C_(max) 1.08 4.28 1.94 0.85 1.82 1.22 (μg/g)/(mg/kg)AUC_(0-4 hr) 1.29 12.38 2.69 1.11 2.19 1.21 (hr * μg/g)/(mg/kg) t_(1/2)(hr) 1.38 4.54 1.55 1.40 1.44 1.05 PM2 C_(max) 1.03 7.46 2.24 1.41 2.401.34 (μg/g)/(mg/kg) AUC_(0-4 hr) 1.35 10.82 2.95 1.36 2.78 1.56 (hr *μg/g)/(mg/kg) t_(1/2) (hr) 1.18 1.27 1.15 1.03 1.08 0.93Comparison of the Formulation and Size Effect on Mbz Disposition BetweenSpecies

Mbz from cosolvent and microemulsions all followed a two-compartmentmodel after i.v. injection. In both mice and rats, Mbz microemulsionsexhibited similar plasma pharmacokinetics as cosolvent. The droplet sizeof Mbz microemulsions did not show significant effect on Mbz plasmapharmacokinetics, as PM1 and PM2 exhibited very similar plasmapharmacokinetics in both mice and rats (Table XV). In Table XVDifferences among groups were statistically evaluated using one-wayANOVA with Turkey's post hoc test at P<0.05. The asterisk * denotesP<0.05 for difference between cosolvent and microemulsion formulations(PM1 and PM2) in rats.

TABLE XV Pharmacokinetic Parameters Cosolvent PM1 PM2 Species Mice RatsMice Rats Mice Rats Dose (mg/kg) 6.5 3.25 3.25 1.6 2.5 1.6 C_(max)/Dose1.45 3.48 ± 0.83 3.49 1.25 ± 0.06 3.24 1.13 ± 0.06 (ug/ml/mg/kg)AUC/Dose 1.14 4.66 ± 0.76 1.22 1.66 ± 0.12 1.44 1.77 ± 0.02 (hr *ug/ml/mg/kg) t_(1/2,) _(—) (hr) 0.078 0.28 ± 0.06 0.068 0.30 ± 0.080.095 0.40 ± 0.07 t_(1/2,) _(—) (hr) 1.34 2.89 ± 1.67 1.52 1.90 ± 0.451.27 2.43 ± 0.36 CL (L/hr/kg) 0.97 0.73 ± 0.1* 0.90 2.03 ± 0.13 0.771.86 ± 0.03 V_(ss) (L/kg) 1.70  2.30 ± 0.90* 1.47 4.50 ± 0.50 1.07 5.27± 0.70 V₁ (L/kg) 0.77  1.00 ± 0.23* 0.30 2.70 ± 0.13 0.33 3.00 ± 0.17 V₂(L/kg) 0.93 1.27 ± 0.83 1.17 1.80 ± 0.63 0.73 2.30 ± 0.57 (hr⁻¹) 8.842.58 ± 0.54 10.01 2.46 ± 0.60 7.28 1.80 ± 0.30 (hr⁻¹) 0.51 0.30 ± 0.121.36 0.36 ± 0.06 0.54 0.30 ± 0.06 k₁₀ (hr⁻¹) 1.27 0.78 ± 0.12 2.87 0.72± 0.06 2.25 0.66 ± 0.02 k₁₂ (hr⁻¹) 4.52 1.08 ± 0.30 6.17 0.78 ± 0.123.81 1.32 ± 0.60 k₂₁ (hr⁻¹) 3.57 1.02 ± 0.36 1.62 1.32 ± 0.60 1.76 0.78± 0.18Development of Pharmacokinetic Models for Dispositions of Mbz inCosolvent and Nanoformulations in Mice

Interestingly, intravenous administration of Mbz microemulsions (PM1 andPM2) resulted in very high exposures and retentions in lung, differentfrom the biodistribution pattern from the cosolvent formulation. Apharmacokinetic model was developed which linked the plasmaconcentrations with lung concentrations of Mbz. A three compartmentalmodel containing central compartment (blood) and two peripheralcompartments (lung and rest of the organs, respectively) was built (FIG.16). The differential equations which described the relationships amongthree compartments were listed as follows:dA ₁ /dt=−(K ₁₂ +K ₁₃ +K ₁₀)*A ₁ +K ₂₁ *A ₂ +K ₃₁ *A ₃  (Eq. 1)dA ₂ /dt=K ₁₂ *A ₁ −K ₂₁ *A ₂  (Eq. 2)dA ₃ /dt=K ₁₃ *A ₁ −K ₃₁ *A ₂  (Eq. 3)where A₁, A₂ and A₃ are the amount of drug in the central, lung andother-organ compartments, respectively. k₁₀ is the elimination ratemicroconstant from the central compartment. k₁₂, k₂₁, k₁₃ and k₃₁, aremicroconstants for the transfers of the drug between the central and theperipheral compartments.

In this model, two outputs including the plasma and lung concentrationsof Mbz were monitored during the studies. Two equations describing theoutputs were listed as follows:C ₁ =A ₁ /V ₁  (Eq. 4)C ₂ =A ₂ *K ₂₁/(V ₁ *K ₁₂)  (Eq. 5)

By fitting experimental data (Table XVI) using ADAPT (FIGS. 17A-17F),the pharmacokinetic parameters in Equations 1-3 were estimated forcosolvent, PM1 and PM2, respectively (Table XVII). Note that the 95%C.I. for cosolvent is unavailable. The AUC Ratio=AUC_(0-6 hr,lung)/Dose:AUC_(0-6,plasma)/Dose.

The estimated k₁₀, k₁₂, k₁₃, and k₃₁ for PM1 were 2.88, 2.99, 8.18, and1.41 hr⁻¹, respectively, similar to those of cosolvent and PM2. Theestimated V₁ was 6.74 L for PM1, also comparable to that of PM2 (9.12L), but much less than that of cosolvent (27.77 L). The estimated k₂₁for PM1 was 1.82 hr⁻¹, much slower than those of PM2 (5.87 hr⁻¹) andcosolvent (5.00 hr⁻¹), which could explain the longer t_(1/2) of Mbz inlung from PM1 (4.54 hr) than those from PM1 (1.27 hr) and cosolvent(1.60 hr). These estimated microconstants could be used to predict Mbzconcentrations in lung from Mbz concentrations in plasma for Mbzmicroemulsions. The ratios of AUC in lung and plasma were 8.64 and 9.13for PM1 and PM2, respectively, much larger than that for cosolvent(2.39).

TABLE XVI Mean plasma concentrations Mean plasma concentrations (μg/ml)(μg/ml) Time Cosolvent PM1 PM2 Time Cosolvent PM1 PM2 (hr) 6.5 mg/kg3.25 mg/kg 2.5 mg/kg (hr) 6.5 mg/kg 3.25 mg/kg 2.5 mg/kg 0.083 5.98 5.873.42 0.083 5.64 13.67 20.01 0.25 3.99 1.93 1.27 0.75 N/A 11.23 8.59 0.52.57 1.37 0.98 2.0 3.05 10.23 4.49 1.0 1.71 1.08 0.68 4.0 1.20 5.67 1.741.5 1.56 0.68 0.64 2.0 1.21 0.53 0.27 3.0 0.79 N/A N/A 4.0 0.54 0.210.07 6.0 0.14 0.10 0.06

A set of Mbz concentrations in plasma and lung, respectively was usedfor validating the identified three-compartment model. Table XVIIprovides the estimated pharmacokinetic parameters of Mbz from cosolvent,PM1 and PM2 for a three-compartment model. The 95% C.I. for cosolvent isunavailable and the AUC ratio is the same. The observed Mbzconcentrations in plasma and lung vs. the predicted Mbz concentrationswere plotted for cosolvent (FIGS. 18A-18B), PM1 (FIGS. 18C-18D) and PM2(FIGS. 18E-18F), respectively. The scattered plot showed that thedeveloped model could predict Mbz concentrations in lung for cosolvent,PM1 and PM2.

TABLE XVII Estimated PK Parameter Values (95% Confidence Interval)Parameter Cosolvent PM1 PM2 k₁₀ (hr⁻¹) 1.10 2.88 3.28 (−9.39, 15.15)(0.66, 5.90) k₁₂(hr⁻¹) 1.55 2.99 3.03 (1.48, 4.51) (2.67, 3.38)k₂₁(hr⁻¹) 5.00 1.82 5.87 (−2.18, 5.83)  (3.02, 8.73) k₁₃(hr⁻¹) 1.57 8.188.35 (−30.18, 46.53)  (−1.83, 18.52) k₃₁ (hr⁻¹) 2.20 1.41 1.97 (−2.59,5.42)  (1.09, 2.84) V₁ (L) 27.77 6.74 9.12 (−18.09, 31.57)   (0.75,17.49) AUC_(0-6 hr,plasma/)Dose 1.08 1.58 1.14 (μg * hr/ml/mg/kg)AUC_(0-6,lung)/Dose 2.57 13.61 10.45  (μg * hr/g/mg/kg) AUC RatioLung/Plasma 2.39 8.64 9.13Prediction of Human Pharmacokinetic Parameters for Mbz from Cosolventand Microemulsions (PM1 and PM2) by Allometric Scaling

The allometric relationships between Mbz pharmacokinetic parameters (CL,V_(ss), t_(1/2), and t_(1/2)) (Table XVII) and body weight forcosolvent, PM1 and PM2 were plotted on a log-log scale (FIGS. 19A-19Fand FIGS. 20A-20F). The 95% of confidence intervals (C.I.) of theregression slopes were displayed. All values are shown as mean±SD.Difference between any two means from one species were statisticallyevaluated using ANOVA, with Tukey's post-hoc analysis. The asterisk *denotes P<0.05 for comparison of pharmacokinetic parameters betweencosolvent and microemulsions in rats.

TABLE XVIII Formulations Cosolvent PM1 PM2 Animal Mice (n = 3) Rats(n-6) Mice (n = 3) Rats (n-3) Mice (n = 3) Rats (n-3) CL (L/hr) 0.032 ±0.006  0.22 ± 0.036* 0.032 ± 0.008 0.61 ± 0.04 0.044 0.56 ± 0.01 (0.036,0.052) Vss (L) 0.057 ± 0.015  0.69 ± 0.27* 0.052 ± 0.015 1.35 ± 0.150.052 1.58 ± 0.21 (0.054, 0.050) t_(1/2), α (hr) 0.08 ± 0.04 0.28 ± 0.060.074 ± 0.027 0.30 ± 0.08 0.060 0.40 ± 0.01 (0.070, 0.050) t_(1/2), β(hr) 1.37 ± 0.11 2.89 ± 1.67 1.51 ± 0.27 1.90 ± 0.45 1.17  2.43 ± 0.36(1.49, 0.86)

The pharmacokinetic parameters in humans predicted from therelationships estimated by inter-species scaling of Mbz from differentmicroemulsion formulations and the 95% C.I. were also compiled in TableXIX. Compared the CL with 95% C.I. (0.09-0.97 L/hr) for cosolvent inhumans, the CL for PM1 (3.05-28.50 L/hr) and PM2 (0.94-10.84 L/hr) werefaster than that for cosolvent. The V. in humans were (9.44-178.57, 95%C.I.) 41.14 L/kg and (26.00-175.75, 95% C.I.) 67.59 L/kg for PM1 andPM2, respectively, which were about 10-20 folds of that for cosolvent.In addition, although the alpha half-lives appeared to be similar amongthese three formulations, the beta half-lives appeared to be distinctbetween cosolvent and microemulsions (PM1 and PM2).

TABLE XIX Formulations PK Parameters CL (L/hr/kg) Vss (L/kg) t1/2, α(hr) t1/2, β (hr) Cosolvent Predictions 0.30  2.95  6.24 22.65  forHumans (0.09, 0.97) (0.04, 29.17) (0.4, 97.72) (0.47, 239.88 Allometric1.65 * 10⁻³ * BW^(0.84) 1.36 * 10⁻³ * BW^(1.07) 0.009 * BW^(0.58) 0.54 *BW^(0.27) Relationships PM1 Predictions 9.33 41.14 15.74 7.94 for Humans(3.05, 28.50) (9.44, 178.57) (2.26, 109.55) (3.42, 18.44) Allometric3.38 * 10⁻⁴ * BW^(1.29) .55 * 10⁻⁴ * BW^(1.43) 0.006 * BW^(0.70) 0.70 *BW^(0.22) Relationships PM1 Predictions 3.20 67.59 12.74 5.20 for Humans(0.94, 10.84) (26.00, 175.75) (2.24, 72.71) (0.61, 44.29) Allometric9.01 * 10⁻⁴ * BW^(1.11) 2.98 * 10⁻⁴ * BW^(1.48) 0.005 * BW^(0.70) 0.56 *BW^(0.20) Relationships

The following references are cited herein.

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Any patents or publications mentioned in this specification areindicative of the levels of those skilled in the art to which theinvention pertains. Further, these patents and publications areincorporated by reference herein to the same extent as if eachindividual publication was specifically and individually incorporated byreference.

What is claimed is:
 1. A drug delivery composition, comprising: a) abenzimidazole derivative having the formula:

wherein R³ is selected from the group consisting of H, carboxyl (—CO₂H),hydroxyl, amino, chloro, difluormethoxy, benzoyl, phenyl-thio,pyridinyl, propyl-thio, diphenyl, 5-methoxy,fluorophenylmethyl-2-chloro, propenyl, chloropropyl or esters (—CO₂R⁴)wherein R⁴ is selected from the group consisting of alkoxy, haloalkyl,alkenyl, and cycloalkyl, wherein the alkyl groups have from 1-8 carbons,or CH₃CH₂(OCH₂CH₂)n-, or CH₃CH₂CH₂(OCH₂CH₂CH₂)n-, or(CH₃)₂CH(OCH(CH₃)CH₂)n-, wherein n is from 1-3 wherein R¹ is OH, Cl, SH,carbamate or piperidin-4-yl, and R² is hydrogen, α-methylvinyl,3-chloropropyl or piperidin-4-yl, or the pharmaceutically effectiveorganic or inorganic salts thereof, or mixtures thereof; b) an oil; c) asurfactant; d) a cosurfactant; and e) a dipolar aprotic solvent, whereinsaid delivery composition forms as a parenterally-deliverablemicroemulsion having a droplet diameter of about 478 nm to less than 500nm.
 2. The drug delivery composition of claim 1, further comprisingwater.
 3. The drug delivery composition of claim 2, wherein water tocomposition weight ratio is about 50%.
 4. The drug delivery compositionof claim 1, wherein the benzimidazole derivative is methyl5-benzoylbenzimidazole-2-carbamate, methyl 5-(phenylthio)-2-carbamate,


5. The drug delivery composition of claim 4, wherein the benzimidazolederivative is methyl 5-benzoylbenzimidazote-2-carbamate at aconcentration of about 0.9 mg/ml to about 2 mg/ml.
 6. The drug deliverycomposition of claim 1, wherein the oil is propylene glycoldicaprylate/dicaprate or triglycerides from coconut oil.
 7. The drugdelivery composition of claim 6, wherein the oil to composition has aweight ratio of about 14% to about 42%.
 8. The drug delivery compositionof claim 1, wherein the surfactant is polysorbate 80 and thecosurfactant is diethylene glycol monoethyl ether or C8/C10mono-/diglyeride.
 9. The drug delivery composition of claim 8, whereinthe surfactant to composition and the cosurfactant to composition haveindividual weight ratios of about 6% to about 48 %.
 10. The drugdelivery composition of claim 9, wherein a ratio ofsurfactant:cosurfactant is about 1:0.5 to about 1:1.
 11. The drugdelivery composition of claim 1, wherein the dipolar aprotic solvent isdimethylsulfoxide.
 12. The drug delivery composition of claim 11,wherein dimethylsulfoxide to composition has a weight ratio of about 5%to about 10%.
 13. A drug delivery composition, comprising: methyl5-benzoylbenzimidazole-2-carbamate; about 4% to about 42% by weight ofan oil; about 20% to about 41% by weight each of a surfactant and acosurfactant in about a 1:0.75 to about 1:1 ratio ofsurfactant:cosurfactant; and about 5% to about 10% by weight ofdimethylsulfoxide, wherein said delivery composition forms aparenterally-deliverable microemulsion having a droplet diameter ofabout 478 nm to less than 500 nm.
 14. The drug delivery composition ofclaim 13, comprising about 4% to about 9% by weight each of propyleneglycol dicaprylate/dicaprate as the oil and about 20% to about 41% byweight of polysorbate 80 as the surfactant and either diethylene glycolmonoethyl ether or C8/C10 mono-/diglyceride as the co-surfactant. 15.The drug delivery composition of claim 14, further comprising about 50%by weight of water.
 16. The drug delivery composition of claim 13,comprising about 42% by weight of propylene glycol dicaprylate/dicaprateor C8/C10 triglycerides from coconut oil as the oil, about 27% by weightof polysorbate 80 combined with diethylene glycol monoethyl ether as thesurfactant and about 21% of by weight of C8/C10 mono-/diglyceride as theco-surfactant.
 17. The drug delivery composition of claim 13, whereinthe methyl 5-benzoylbenzimidazole-2-carbamate is at a concentration ofabout 0.9mg/ml to about 2 mg/ml.